Cell Biology Antibodies 16
Anti-Phospho-MAP2K1-S217/MAP2K2-S221 Antibody (CABP0209)
(No reviews yet)
Write a Review
- SKU:
- CABP0209
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-MAP2K1-S217/MAP2K2-S221 Antibody |
| Antibody SKU: | CABP0209 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding serine 217/221 of human MAP2K1/MAP2K2. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding serine 217/221 of human MAP2K1/MAP2K2. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5604/5605 |
| Uniprot: | Q02750/P36507 |
| Cellular Location: | |
| Calculated MW: | 40kDa/43kDa/44kDa |
| Observed MW: | 44kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S217/MAP2K2-S221 antibody (CABP0209) at dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37'C for 15 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
| Synonyms: | MEK1/MEK2 |
| Background: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
Related Products
Phospho-MEK1/MEK2-S217/S221 Monoclonal Antibody
OverviewProduct Name:Phospho-MEK1/MEK2-S217/S221 Monoclonal AntibodyProduct Code:CABP1349Reactivity:Human, MouseApplications:West
Human/Mouse/Rat Phospho-MEK1 (S217/S221) PharmaGenie ELISA Kit (SBRS1891)
Human/Mouse/Rat Phospho-MEK1 (S217/S221) PharmaGenie ELISA Kit (SBRS1891)OverviewProduct SKU:SBRS1891Size:96TApplication:The antibody pair provided in thi
Human/Mouse/Rat Phospho-MEK1 (S217/S221) and Total Mek PharmaGenie ELISA Kit (SBRS1892)
Human/Mouse/Rat Phospho-MEK1 (S217/S221) and Total Mek PharmaGenie ELISA Kit (SBRS1892)OverviewProduct SKU:SBRS1892Size:96TApplication:The antibody pair p