Cell Biology Antibodies 16
Anti-Phospho-LIM domain kinase 2-T505 Antibody (CABP0388)
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- SKU:
- CABP0388
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-LIM domain kinase 2-T505 Antibody |
| Antibody SKU: | CABP0388 |
| Antibody Size: | 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3985 |
| Uniprot: | P53671 |
| Cellular Location: | Cytoplasm, Nucleus, Nucleus |
| Calculated MW: | 69kDa/72kDa/77kDa |
| Observed MW: | 72kDa |
 | Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody (CABP0388). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-LIMK2-T505 antibody (CABP0388). |
 | Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-LIMK2-T505 antibody (CABP0388). |
| Synonyms: | LIMK2 |
| Background: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified. |
| UniProt Protein Details: | Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cis-Golgi network; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation |
| NCBI Summary: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P53671 |
| NCBI GenInfo Identifier: | 1708824 |
| NCBI Gene ID: | 3985 |
| NCBI Accession: | P53671.1 |
| UniProt Secondary Accession: | P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5 |
| UniProt Related Accession: | P53671 |
| Molecular Weight: | 638 |
| NCBI Full Name: | LIM domain kinase 2 |
| NCBI Synonym Full Names: | LIM domain kinase 2 |
| NCBI Official Symbol: | LIMK2 |
| NCBI Protein Information: | LIM domain kinase 2 |
| UniProt Protein Name: | LIM domain kinase 2 |
| Protein Family: | LIM domain kinase |
| UniProt Gene Name: | LIMK2 |
| UniProt Entry Name: | LIMK2_HUMAN |
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