Cell Biology Antibodies 16

Anti-Phospho-Lck-Y416/FYN-Y416 Antibody (CABP0491)

(No reviews yet) Write a Review
SKU:
CABP0491

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-Lck-Y416/FYN-Y416 Antibody
Antibody SKU:CABP0491
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:Human
Positive Samples:Jurkat
Immunogen:A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2534/3055/3932/4067/6714/7525
Uniprot:P06241/P08631/P06239/P07948/P12931/P07947
Cellular Location:
Calculated MW:40kDa/58kDa/61kDa/54kDa/60kDa
Observed MW:56KDa
Anti-Phospho-Lck-Y416/FYN-Y416 Antibody (CABP0491)Western blot analysis of extracts of Jurkat cells, using Phospho-SRC Family-Y416 antibody (CABP0491) at 1:1000 dilution.Jurkat cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00070). Exposure time: .
Anti-Phospho-Lck-Y416/FYN-Y416 Antibody (CABP0491)Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-SRC Family-Y416 pAb (CABP0491). Western blot was performed from the immunoprecipitate using Anti-Phospho-Lck-Y416/FYN-Y416 Antibody (CABP0491) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37'C for 2 minutes after serum-starvation overnight.
Synonyms:ASV, SRC1, THC6, c-SRC, p60-Src, SRC
Background:This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
View AllClose

Additional Information

Product Type:
Antibody
Applications:
WB
Applications:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
View AllClose

Related Products