Cell Biology Antibodies 16

Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)

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SKU:
CABP0631
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody
Antibody SKU:CABP0631
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, C6
Immunogen:A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5599/5601/5602
Uniprot:P45983/P45984/P53779
Cellular Location:
Calculated MW:35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW:46KDa/56KDa
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (CABP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37'C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens).
Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.
Synonyms:JNK1/JNK2/JNK3
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
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