Cell Biology Antibodies 16

Anti-Phospho-IKK alpha/beta-S176/180 pAb Antibody (CABP0891)

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SKU:
CABP0891
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Phospho-IKK alpha/beta-S176/180 Antibody
Antibody SKU:CABP0891
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding S176/180 of human IKKAlpha/Beta.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:A phospho specific peptide corresponding to residues surrounding S176/180 of human IKKAlpha/Beta.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1147/3551
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:85kDa
Anti-Phospho-IKK alpha/beta-S176/180 pAb (CABP0891)Western blot analysis of extracts of HeLa cells, using Phospho-IKKa/b-S176/180 antibody (CABP0891) at 1:1000 dilution.HeLa cells were treated by TNF-a (20 ng/mL) and Calyculin A (100 nM) at 37'C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:
Background:This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
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