Immunology Antibodies 3
Anti-Phospho-HSPB1-S82 Antibody (CABP0041)
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- SKU:
- CABP0041
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-HSPB1-S82 Antibody |
| Antibody SKU: | CABP0041 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S82 of human HSPB1 (NP_001531.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S82 of human HSPB1 (NP_001531.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QLSS G |
| Gene ID: | 3315 |
| Uniprot: | P04792 |
| Cellular Location: | Cytoplasm, Nucleus, cytoskeleton, spindle |
| Calculated MW: | 22kDa |
| Observed MW: | 28kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 antibody (CABP0041) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
 | Western blot analysis of extracts of HeLa cells, using Anti-Phospho-HSPB1-S82 Antibody (CABP0041) at 1:1000 dilution or HSPB1 antibody (CAB16332).HeLa cells were treated by UV at room temperature for 15-30 minutes.HeLa cells were treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using Phospho-HSPB1-S82 antibody (CABP0041). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Anti-Phospho-HSPB1-S82 Antibody (CABP0041). Western blot was performed from the immunoprecipitate using Anti-Phospho-HSPB1-S82 Antibody (CABP0041) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. |
| Synonyms: | HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 |
| Background: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). |
| UniProt Protein Function: | HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. |
| UniProt Protein Details: | Protein type:Chaperone; Motility/polarity/chemotaxis; Heat shock protein Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; nucleus; cytosol Molecular Function:identical protein binding; protein kinase C inhibitor activity; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding Biological Process: positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; retinal homeostasis; positive regulation of angiogenesis; response to virus; positive regulation of blood vessel endothelial cell migration; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein; negative regulation of apoptosis; regulation of translational initiation Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f |
| NCBI Summary: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| UniProt Code: | P04792 |
| NCBI GenInfo Identifier: | 19855073 |
| NCBI Gene ID: | 3315 |
| NCBI Accession: | P04792.2 |
| UniProt Secondary Accession: | P04792,Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35 Q9UC36, B2R4N8, |
| UniProt Related Accession: | P04792 |
| Molecular Weight: | 205 |
| NCBI Full Name: | Heat shock protein beta-1 |
| NCBI Synonym Full Names: | heat shock 27kDa protein 1 |
| NCBI Official Symbol: | HSPB1 |
| NCBI Official Synonym Symbols: | CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102 |
| NCBI Protein Information: | heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; epididymis secretory protein Li 102 |
| UniProt Protein Name: | Heat shock protein beta-1 |
| UniProt Synonym Protein Names: | 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
| Protein Family: | Heat shock protein |
| UniProt Gene Name: | HSPB1 |
| UniProt Entry Name: | HSPB1_HUMAN |
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