Immunology Antibodies 3

Anti-Phospho-HSPB1-S15 Antibody (CABP0040)

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SKU:
CABP0040
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Phospho-HSPB1-S15 Antibody
Antibody SKU:CABP0040
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S15 of human HSPB1 (NP_001531.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human
Positive Samples:HeLa
Immunogen:A synthetic phosphorylated peptide around S15 of human HSPB1 (NP_001531.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GPSW D
Gene ID:3315
Uniprot:P04792
Cellular Location:Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:22kDa
Observed MW:28kDa
Anti-Phospho-HSPB1-S15 Antibody (CABP0040)Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S15 antibody (CABP0040) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Anti-Phospho-HSPB1-S15 Antibody (CABP0040)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Anti-Phospho-HSPB1-S15 Antibody (CABP0040). Western blot was performed from the immunoprecipitate using Anti-Phospho-HSPB1-S15 Antibody (CABP0040) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight.
Synonyms:HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Background:The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
UniProt Protein Function:HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
UniProt Protein Details:

Protein type:Chaperone; Motility/polarity/chemotaxis; Heat shock protein

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; nucleus; cytosol

Molecular Function:identical protein binding; protein kinase C inhibitor activity; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding

Biological Process: positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; retinal homeostasis; positive regulation of angiogenesis; response to virus; positive regulation of blood vessel endothelial cell migration; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein; negative regulation of apoptosis; regulation of translational initiation

Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f

NCBI Summary:The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
UniProt Code:P04792
NCBI GenInfo Identifier:19855073
NCBI Gene ID:3315
NCBI Accession:P04792.2
UniProt Secondary Accession:P04792,Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35 Q9UC36, B2R4N8,
UniProt Related Accession:P04792
Molecular Weight:205
NCBI Full Name:Heat shock protein beta-1
NCBI Synonym Full Names:heat shock 27kDa protein 1
NCBI Official Symbol:HSPB1  
NCBI Official Synonym Symbols:CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102  
NCBI Protein Information:heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; epididymis secretory protein Li 102
UniProt Protein Name:Heat shock protein beta-1
UniProt Synonym Protein Names:28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Protein Family:Heat shock protein
UniProt Gene Name:HSPB1  
UniProt Entry Name:HSPB1_HUMAN
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