Cell Biology Antibodies 16
Anti-Phospho-GAB1-Y659 pAb Antibody (CABP0774)
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- SKU:
- CABP0774
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-GAB1-Y659 Antibody |
| Antibody SKU: | CABP0774 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VDYV V |
| Gene ID: | 2549 |
| Uniprot: | Q13480 |
| Cellular Location: | |
| Calculated MW: | 76kDa/80kDa |
| Observed MW: | 110kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-GAB1-Y659 antibody (CABP0774) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | GAB1, GRB2 |
| Background: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GAB1: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Belongs to the GAB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q31.21 Cellular Component: cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of JNK activity; cell proliferation; epidermal growth factor receptor signaling pathway; epidermis development; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; regulation of cell migration; response to oxidative stress |
| NCBI Summary: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q13480 |
| NCBI GenInfo Identifier: | 90180201 |
| NCBI Gene ID: | 2549 |
| NCBI Accession: | Q13480.2 |
| UniProt Secondary Accession: | Q13480,Q4W5G2, Q6P1W2, A8K152, |
| UniProt Related Accession: | Q13480 |
| Molecular Weight: | 80,005 Da |
| NCBI Full Name: | GRB2-associated-binding protein 1 |
| NCBI Synonym Full Names: | GRB2 associated binding protein 1 |
| NCBI Official Symbol: | GAB1 |
| NCBI Protein Information: | GRB2-associated-binding protein 1 |
| UniProt Protein Name: | GRB2-associated-binding protein 1 |
| UniProt Synonym Protein Names: | GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1 |
| Protein Family: | GPI transamidase component |
| UniProt Gene Name: | GAB1 |
| UniProt Entry Name: | GAB1_HUMAN |
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