Cell Cycle Antibodies 2
Anti-Phospho-FOXO4-S197 Antibody (CABP0177)
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- SKU:
- CABP0177
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Phospho-FOXO4-S197 Antibody |
| Antibody SKU: | CABP0177 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S197 of human FOXO4 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S197 of human FOXO4 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4303 |
| Uniprot: | P98177 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 47kDa/53kDa |
| Observed MW: | 60kDa |
 | Western blot analysis of extracts from 293 cells using phospho-FOXO4-S197 antibody (CABP0177). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-FOXO4-S197 antibody (CABP0177). |
 | Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-FOXO4-S197 antibody (CABP0177). |
| Synonyms: | AFX, AFX1, MLLT7, FOXO4 |
| Background: | This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Oncoprotein; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: Xq13.1 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:beta-catenin binding; DNA binding; enzyme binding; identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: cell cycle arrest; insulin receptor signaling pathway; negative regulation of angiogenesis; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; stem cell differentiation; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P98177 |
| NCBI GenInfo Identifier: | 110825720 |
| NCBI Gene ID: | 4303 |
| NCBI Accession: | P98177.5 |
| UniProt Secondary Accession: | P98177,O43821, Q13720, Q3KPF1, Q8TDK9, B7WPJ7, |
| UniProt Related Accession: | P98177 |
| Molecular Weight: | 47,941 Da |
| NCBI Full Name: | Forkhead box protein O4 |
| NCBI Synonym Full Names: | forkhead box O4 |
| NCBI Official Symbol: | FOXO4 |
| NCBI Official Synonym Symbols: | AFX; AFX1; MLLT7 |
| NCBI Protein Information: | forkhead box protein O4 |
| UniProt Protein Name: | Forkhead box protein O4 |
| UniProt Synonym Protein Names: | Fork head domain transcription factor AFX1 |
| Protein Family: | Forkhead box protein |
| UniProt Gene Name: | FOXO4 |
| UniProt Entry Name: | FOXO4_HUMAN |
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