Cell Biology Antibodies 16
Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 pAb Antibody (CABP0892)
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- SKU:
- CABP0892
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 Antibody |
| Antibody SKU: | CABP0892 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T24/T32/T28 of human FoxO1/FoxO3a/FoxO4. |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, NIH/3T3 |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T24/T32/T28 of human FoxO1/FoxO3a/FoxO4. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2308/2309/4303 |
| Uniprot: | Q12778/O43524 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 65kDa/78kDa |
 | Western blot analysis of extracts of Jurkat cells, using Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 pAb (CABP0892) at 1:1000 dilution.Jurkat cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of NIH/3T3 cells, using Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 pAb (CABP0892) at 1:1000 dilution or FOXO1 antibody (CAB13862).NIH/3T3 cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat liver using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |
| Synonyms: | FoxO1/FoxO3a/FoxO4 |
| Background: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
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