Cell Cycle Antibodies 2

Anti-Phospho-ERK3-S189 Antibody (CABP0051)

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SKU:
CABP0051

Description

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Antibody Name:Anti-Phospho-ERK3-S189 Antibody
Antibody SKU:CABP0051
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293
Immunogen:A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLSE G
Gene ID:5597
Uniprot:Q16659
Cellular Location:Cytoplasm, Nucleus
Calculated MW:82kDa
Observed MW:120kDa
Anti-Phospho-ERK3-S189 Antibody (CABP0051)Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (CABP0051) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Synonyms:MAPK6, ERK3, HsT17250, PRKM6, p97MAPK
Background:The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
UniProt Protein Function:ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:MAP kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: protein amino acid phosphorylation; signal transduction

NCBI Summary:The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
UniProt Code:Q16659
NCBI GenInfo Identifier:2499596
NCBI Gene ID:5597
NCBI Accession:Q16659.1
UniProt Secondary Accession:Q16659,Q68DH4, Q8IYN8, B2R945, B5BU65,
UniProt Related Accession:Q16659
Molecular Weight:82,681 Da
NCBI Full Name:Mitogen-activated protein kinase 6
NCBI Synonym Full Names:mitogen-activated protein kinase 6
NCBI Official Symbol:MAPK6  
NCBI Official Synonym Symbols:ERK3; PRKM6; p97MAPK; HsT17250  
NCBI Protein Information:mitogen-activated protein kinase 6
UniProt Protein Name:Mitogen-activated protein kinase 6
UniProt Synonym Protein Names:Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
UniProt Gene Name:MAPK6  
UniProt Entry Name:MK06_HUMAN
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Additional Information

Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
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