Cardiovascular Antibodies
Anti-Phospho-EphA2-Y594 pAb Antibody (CABP0818)
- SKU:
- CABP0818
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-Phospho-EphA2-Y594 Antibody |
| Antibody SKU: | CABP0818 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | SH-SY5Y |
| Immunogen: | A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HTYE D |
| Gene ID: | 1969 |
| Uniprot: | P29317 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane, ruffle membrane |
| Calculated MW: | 54kDa/108kDa |
| Observed MW: | 108kDa |
 | Western blot analysis of extracts of SH-SY5Y cells, using Phospho-EphA2-Y594 antibody (CABP0818) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/mL) for 5 minutes . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| Synonyms: | ARCC2, CTPA, CTPP1, CTRCT6, ECK, EPHA2 |
| Background: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders. |
| UniProt Protein Function: | EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice. |
| UniProt Protein Details: | Protein type:Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Eph family Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cell surface; focal adhesion; integral to plasma membrane; intracellular; plasma membrane Molecular Function:ATP binding; ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity Biological Process: angiogenesis; axial mesoderm formation; axon guidance; bone remodeling; cell adhesion; cell migration; DNA damage response, signal transduction resulting in induction of apoptosis; ephrin receptor signaling pathway; keratinocyte differentiation; mammary gland epithelial cell proliferation; multicellular organismal development; negative regulation of protein kinase B signaling cascade; neural tube development; notochord cell development; notochord formation; osteoblast differentiation; osteoclast differentiation; peptidyl-tyrosine phosphorylation; protein kinase B signaling cascade; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; skeletal development; vasculogenesis; viral reproduction Disease: Cataract 6, Multiple Types |
| NCBI Summary: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010] |
| UniProt Code: | P29317 |
| NCBI GenInfo Identifier: | 229462861 |
| NCBI Gene ID: | 1969 |
| NCBI Accession: | P29317.2 |
| UniProt Secondary Accession: | P29317,Q8N3Z2, B5A968, |
| UniProt Related Accession: | P29317 |
| Molecular Weight: | |
| NCBI Full Name: | Ephrin type-A receptor 2 |
| NCBI Synonym Full Names: | EPH receptor A2 |
| NCBI Official Symbol: | EPHA2 |
| NCBI Official Synonym Symbols: | ECK; CTPA; ARCC2; CTPP1; CTRCT6 |
| NCBI Protein Information: | ephrin type-A receptor 2 |
| UniProt Protein Name: | Ephrin type-A receptor 2 |
| UniProt Synonym Protein Names: | Epithelial cell kinase; Tyrosine-protein kinase receptor ECK |
| Protein Family: | Ephrin type-A receptor |
| UniProt Gene Name: | EPHA2 |
| UniProt Entry Name: | EPHA2_HUMAN |
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