Signal Transduction Antibodies 3
Anti-Phospho-EIF4EBP1-T70 Antibody (CABP0031)
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- SKU:
- CABP0031
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-Phospho-EIF4EBP1-T70 Antibody |
| Antibody SKU: | CABP0031 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T70 of human EIF4EBP1 (NP_004086.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around T70 of human EIF4EBP1 (NP_004086.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TKTP P |
| Gene ID: | 1978 |
| Uniprot: | Q13541 |
| Cellular Location: | |
| Calculated MW: | 12kDa |
| Observed MW: | 17kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-EIF4EBP1-T70 antibody (CABP0031) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37'C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
| Synonyms: | 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4EBP1 |
| Background: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| UniProt Protein Function: | 4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 8p12 Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol Molecular Function:protein binding; translation repressor activity; eukaryotic initiation factor 4E binding Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development |
| NCBI Summary: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13541 |
| NCBI GenInfo Identifier: | 34921508 |
| NCBI Gene ID: | 1978 |
| NCBI Accession: | Q13541.3 |
| UniProt Secondary Accession: | Q13541,Q6IBN3, B2R502, D3DSW8, |
| UniProt Related Accession: | Q13541 |
| Molecular Weight: | 118 |
| NCBI Full Name: | Eukaryotic translation initiation factor 4E-binding protein 1 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E binding protein 1 |
| NCBI Official Symbol: | EIF4EBP1 |
| NCBI Official Synonym Symbols: | BP-1; 4EBP1; 4E-BP1; PHAS-I |
| NCBI Protein Information: | eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4E-binding protein 1 |
| UniProt Synonym Protein Names: | Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
| Protein Family: | Eukaryotic translation initiation factor 4E-binding protein |
| UniProt Gene Name: | EIF4EBP1 |
| UniProt Entry Name: | 4EBP1_HUMAN |
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