ChIP Antibodies
Anti-Phospho-eIF4E-S209 Antibody (CABP0747)
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- SKU:
- CABP0747
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-eIF4E-S209 Antibody |
| Antibody SKU: | CABP0747 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S209 of human eIF4E |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HT-29, NIH/3T3 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S209 of human eIF4E |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1977 |
| Uniprot: | P06730 |
| Cellular Location: | Cytoplasm, P-body |
| Calculated MW: | 25kDa/27kDa/28kDa |
| Observed MW: | 25kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eIF4E-S209 antibody (CABP0747) at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
| Synonyms: | AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, EIF4E |
| Background: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 4q23 Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol Molecular Function:protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development Disease: Autism, Susceptibility To, 19 |
| NCBI Summary: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P06730 |
| NCBI GenInfo Identifier: | 1352435 |
| NCBI Gene ID: | 1977 |
| NCBI Accession: | P06730.2 |
| UniProt Secondary Accession: | P06730,Q96E95, B7Z6V1, D6RCQ6, |
| UniProt Related Accession: | P06730 |
| Molecular Weight: | 27,260 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 4E |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E |
| NCBI Official Symbol: | EIF4E |
| NCBI Official Synonym Symbols: | CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1 |
| NCBI Protein Information: | eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4E |
| UniProt Synonym Protein Names: | eIF-4F 25 kDa subunit; mRNA cap-binding protein |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4E |
| UniProt Entry Name: | IF4E_HUMAN |
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