Metabolism Antibodies 3
Anti-Phospho-eIF2alpha-S51 Antibody (CABP0745)
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- SKU:
- CABP0745
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-Phospho-eIF2alpha-S51 Antibody |
| Antibody SKU: | CABP0745 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S51 of human eIF2Alpha (NP_004085.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:1000 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S51 of human eIF2Alpha (NP_004085.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SELS R |
| Gene ID: | 1965 |
| Uniprot: | P05198 |
| Cellular Location: | Cytoplasmic granule |
| Calculated MW: | 36kDa |
| Observed MW: | 38kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-eIF2a-S51 pAb (CABP0745) at 1:2000 dilution or EIF2S1 antibody (CAB0764).HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2a-S51 pAb (CABP0745). Western blot was performed from the immunoprecipitate using Phospho-eIF2a-S51 pAb (CABP0745) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37'C for 30 minutes after serum-starvation overnight. |
| Synonyms: | EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1 |
| Background: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| UniProt Protein Function: | eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation initiation; Translation Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex Molecular Function:protein binding; translation initiation factor activity; ribosome binding Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression |
| NCBI Summary: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| UniProt Code: | P05198 |
| NCBI GenInfo Identifier: | 124200 |
| NCBI Gene ID: | 1965 |
| NCBI Accession: | P05198.3 |
| UniProt Related Accession: | P05198 |
| Molecular Weight: | 315 |
| NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 1 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
| NCBI Official Symbol: | EIF2S1 |
| NCBI Official Synonym Symbols: | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha |
| NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha |
| UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 1 |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF2S1 |
| UniProt Entry Name: | IF2A_HUMAN |
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