Metabolism Antibodies 3

Anti-Phospho-eIF2a-S49 Antibody (CABP0341)

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SKU:
CABP0341
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-eIF2a-S49 Antibody
Antibody SKU:CABP0341
Antibody Size:50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding S49 of human EIF2S1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:A phospho specific peptide corresponding to residues surrounding S49 of human EIF2S1
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1965
Uniprot:P05198
Cellular Location:Cytoplasmic granule
Calculated MW:36kDa
Observed MW:40kDa
Anti-Phospho-eIF2a-S49 Antibody (CABP0341)Western blot analysis of extracts from HeLa cells, using phospho-EIF2S1-S49 antibody (CABP0341). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Anti-Phospho-eIF2a-S49 Antibody (CABP0341)Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-eIF2a-S49 antibody (CABP0341).
Synonyms:EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1
Background:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Protein Function:eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function:protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression

NCBI Summary:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Code:P05198
NCBI GenInfo Identifier:124200
NCBI Gene ID:1965
NCBI Accession:P05198.3
UniProt Related Accession:P05198
Molecular Weight:315
NCBI Full Name:Eukaryotic translation initiation factor 2 subunit 1
NCBI Synonym Full Names:eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol:EIF2S1  
NCBI Official Synonym Symbols:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha  
NCBI Protein Information:eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
UniProt Protein Name:Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF2S1  
UniProt Entry Name:IF2A_HUMAN
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