Cell Biology Antibodies 16
Anti-Phospho-CTTN-Y466 Antibody (CABP0247)
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- SKU:
- CABP0247
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-CTTN-Y466 Antibody |
| Antibody SKU: | CABP0247 |
| Antibody Size: | 100 uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y466 of human CTTN |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y466 of human CTTN |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2017 |
| Uniprot: | Q14247 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton, dendrite, dendritic spine, focal adhesion, lamellipodium, podosome, ruffle |
| Calculated MW: | 57kDa/61kDa/70kDa |
| Observed MW: | 80kDa |
 | Western blot analysis of extracts from Jurkat cells using Phospho-CTTN-Y466 antibody (CABP0247). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | CTTN, EMS1, cortactin |
| Background: | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. |
| UniProt Protein Function: | Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified. |
| UniProt Protein Details: | Protein type:Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13 Cellular Component: actin filament; cell cortex; coated pit; cortical cytoskeleton; cytoplasm; cytoskeleton; focal adhesion; Golgi apparatus; growth cone; intracellular membrane-bound organelle; lamellipodium; plasma membrane; ruffle; voltage-gated potassium channel complex Molecular Function:profilin binding; protein binding Biological Process: actin cytoskeleton reorganization; cell motility involved in cell locomotion; focal adhesion formation; intracellular protein transport; neurite morphogenesis; positive regulation of actin filament polymerization; positive regulation of smooth muscle contraction; receptor-mediated endocytosis; regulation of axon extension |
| NCBI Summary: | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | Q14247 |
| NCBI GenInfo Identifier: | 215273892 |
| NCBI Gene ID: | 2017 |
| NCBI Accession: | Q14247.2 |
| UniProt Secondary Accession: | Q14247,Q8N707, Q96H99, |
| UniProt Related Accession: | Q14247,AAB26248 |
| Molecular Weight: | 57,467 Da |
| NCBI Full Name: | Src substrate cortactin |
| NCBI Synonym Full Names: | cortactin |
| NCBI Official Symbol: | CTTN |
| NCBI Official Synonym Symbols: | EMS1 |
| NCBI Protein Information: | src substrate cortactin |
| UniProt Protein Name: | Src substrate cortactin |
| UniProt Synonym Protein Names: | Amplaxin; Oncogene EMS1 |
| Protein Family: | CTTNBP2 N-terminal-like protein |
| UniProt Gene Name: | CTTN |
| UniProt Entry Name: | SRC8_HUMAN |
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