Cell Biology Antibodies 16
Anti-Phospho-CRKL-Y207 pAb Antibody (CABP0824)
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- SKU:
- CABP0824
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-CRKL-Y207 Antibody |
| Antibody SKU: | CABP0824 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562 |
| Immunogen: | A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HAYA Q |
| Gene ID: | 1399 |
| Uniprot: | P46109 |
| Cellular Location: | |
| Calculated MW: | 33kDa |
| Observed MW: | 38kDa |
 | Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody (CABP0824) at 1:1000 dilution or CRKL antibody (CAB11735). K-562 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat brain using Phospho-CRKL-Y207 antibody (CABP0824) at dilution of 1:100 (40x lens). |
| Synonyms: | CRKL |
| Background: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| UniProt Protein Function: | CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; endosome Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of MAPKK activity; JNK cascade; positive regulation of cell proliferation; Ras protein signal transduction |
| NCBI Summary: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009] |
| UniProt Code: | P46109 |
| NCBI GenInfo Identifier: | 1169094 |
| NCBI Gene ID: | 1399 |
| NCBI Accession: | P46109.1 |
| UniProt Secondary Accession: | P46109,A8KA44, D3DX35, |
| UniProt Related Accession: | P46109 |
| Molecular Weight: | 33,777 Da |
| NCBI Full Name: | Crk-like protein |
| NCBI Synonym Full Names: | CRK like proto-oncogene, adaptor protein |
| NCBI Official Symbol: | CRKL |
| NCBI Protein Information: | crk-like protein |
| UniProt Protein Name: | Crk-like protein |
| Protein Family: | Crk-like protein |
| UniProt Gene Name: | CRKL |
| UniProt Entry Name: | CRKL_HUMAN |
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