Cell Biology Antibodies 15
Anti-Phospho-CGAS-Y215 Antibody (CABP0946)
(No reviews yet)
Write a Review
- SKU:
- CABP0946
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Description
| Antibody Name: | Anti-Phospho-CGAS-Y215 Antibody |
| Antibody SKU: | CABP0946 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y215 of human CGAS. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, NIH/3T3, C6 |
| Immunogen: | A synthetic phosphorylated peptide around Y215 of human CGAS. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 115004 |
| Uniprot: | Q8N884 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 59kDa |
| Observed MW: | 59kDa |
 | Western blot - Phospho-CGAS-Y215 Rabbit pAb (CABP0946) |
| Synonyms: | MB21D1, C6orf150, cGAS, h-cGAS, CGAS |
| Background: | Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity (PubMed:23258413, PubMed:23707061, PubMed:23722159, PubMed:24077100, PubMed:25131990, PubMed:29976794, PubMed:30799039). Catalysis involves both the formation of a 2', 5' phosphodiester linkage at the GpA step and the formation of a 3', 5' phosphodiester linkage at the ApG step, producing c[G(2', 5')pA(3', 5')p] (PubMed:28363908, PubMed:28214358). Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses (PubMed:28363908). Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production (PubMed:28363908, PubMed:28314590). Preferentially recognizes and binds curved long DNAs (PubMed:30007416). In contrast to other mammals, human CGAS displays species-specific mechanisms of DNA recognition and produces less cyclic GMP-AMP (cGAMP), allowing a more fine-tuned response to pathogens (PubMed:30007416). Has antiviral activity by sensing the presence of dsDNA from DNA viruses in the cytoplasm (PubMed:28363908). Also acts as an innate immune sensor of infection by retroviruses, such as HIV-1, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:23929945). Detection of retroviral reverse-transcribed DNA in the cytosol may be indirect and be mediated via interaction with PQBP1, which directly binds reverse-transcribed retroviral DNA (PubMed:26046437). Also detects the presence of DNA from bacteria, such as M.tuberculosis (PubMed:26048138). cGAMP can be transferred from producing cells to neighboring cells through gap junctions, leading to promote TMEM173/STING activation and convey immune response to connecting cells (PubMed:24077100). |
| UniProt Protein Function: | MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. |
| UniProt Protein Details: | Protein type:EC 2.7.7.86; Enzyme, cellular metabolism Chromosomal Location of Human Ortholog: 6q13 Cellular Component: cytosol Molecular Function:DNA binding; GTP binding; metal ion binding; ATP binding Biological Process: cyclic nucleotide biosynthetic process; positive regulation of interferon type I production; innate immune response; positive regulation of defense response to virus by host; defense response to virus; activation of innate immune response |
| UniProt Code: | Q8N884 |
| NCBI GenInfo Identifier: | 115511030 |
| NCBI Gene ID: | 115004 |
| NCBI Accession: | NP_612450.2 |
| UniProt Secondary Accession: | Q8N884,Q14CV6, Q32NC9, Q5SWL0, Q5SWL1, Q96E45, L0L2J9 |
| UniProt Related Accession: | Q8N884 |
| Molecular Weight: | 58.3 kDa |
| NCBI Full Name: | cyclic GMP-AMP synthase |
| NCBI Synonym Full Names: | Mab-21 domain containing 1 |
| NCBI Official Symbol: | MB21D1 |
| NCBI Official Synonym Symbols: | cGAS; h-cGAS; C6orf150 |
| NCBI Protein Information: | cyclic GMP-AMP synthase; cGAMP synthase; protein MB21D1; mab-21 domain-containing protein 1 |
| UniProt Protein Name: | Cyclic GMP-AMP synthase |
| UniProt Synonym Protein Names: | Mab-21 domain-containing protein 1 |
| UniProt Gene Name: | MB21D1 |
| UniProt Entry Name: | CGAS_HUMAN |
Related Products
Anti-Phospho-CGAS-S291 Antibody (CABP1176)
OverviewProduct Name:Phospho-CGAS-S291 Rabbit pAbProduct Code:CABP1176Size:20uL, 50uL, 100uLSynonyms:E330016A19Rik, cGAS
Anti-Phospho-CGAS-S420 Antibody (CABP1228)
OverviewProduct Name:Phospho-CGAS-S420 Rabbit pAbProduct Code:CABP1228Size:50uL, 100uLSynonyms:E330016A19Rik, cGASAppl
Anti-Phospho-Cgas-S420 Antibody (CABP1261)
OverviewProduct Name:Phospho-Cgas-S420 Rabbit pAbProduct Code:CABP1261Size:50uL, 100uLSynonyms:E330016A19Rik, cGASAppl
Anti-cGAS Antibody (CAB20125)
OverviewProduct Name:cGAS Rabbit pAbProduct Code:CAB20125Size:20uL, 50uL, 100uLSynonyms:MB21D1, C6orf150, cGAS, h-cGAS, CGAS