Cell Cycle Antibodies 2

Anti-Phospho-cdc25C-S216 Antibody (CABP0577)

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SKU:
CABP0577
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-Phospho-cdc25C-S216 Antibody
Antibody SKU:CABP0577
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HT-29
Immunogen:A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPSM P
Gene ID:995
Uniprot:P30307
Cellular Location:Nucleus
Calculated MW:45kDa/48kDa/50kDa/53kDa
Observed MW:53kDa
Anti-Phospho-cdc25C-S216 Antibody (CABP0577)Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody (CABP0577). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Synonyms:CDC25C, CDC25, PPP1R60
Background:This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
UniProt Protein Function:CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.
UniProt Protein Details:

Protein type:EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:phosphoprotein phosphatase activity; protein binding; protein kinase binding; WW domain binding

Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA replication; G2/M transition of mitotic cell cycle; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitosis

NCBI Summary:This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
UniProt Code:P30307
NCBI GenInfo Identifier:116242631
NCBI Gene ID:995
NCBI Accession:P30307.2
UniProt Secondary Accession:P30307,Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1, D3DQB8
UniProt Related Accession:P30307
Molecular Weight:45,608 Da
NCBI Full Name:M-phase inducer phosphatase 3
NCBI Synonym Full Names:cell division cycle 25C
NCBI Official Symbol:CDC25C  
NCBI Official Synonym Symbols:CDC25; PPP1R60  
NCBI Protein Information:M-phase inducer phosphatase 3
UniProt Protein Name:M-phase inducer phosphatase 3
UniProt Synonym Protein Names:Dual specificity phosphatase Cdc25C
UniProt Gene Name:CDC25C  
UniProt Entry Name:MPIP3_HUMAN
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