Antibody Name:	Anti-Phospho-CAD-S1859 Antibody
Antibody SKU:	CABP0884
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic phosphorylated peptide around S1859 of human CAD (NP_004332.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:

Immunogen:	A synthetic phosphorylated peptide around S1859 of human CAD (NP_004332.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RASD P
Gene ID:	790
Uniprot:	P27708
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	242kDa
Observed MW:	Refer to figures

Synonyms:

CAD, CDG1Z, EIEE50, GATD4

Background:

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.
UniProt Protein Details:	Protein type:EC 3.5.2.3; EC 6.3.5.5; Nucleotide Metabolism - pyrimidine; Amino Acid Metabolism - alanine, aspartate and glutamate; Ligase; Hydrolase; Transferase; EC 2.1.3.2 Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: cell projection; cell soma; cytosol; membrane; nuclear matrix; nucleoplasm; nucleus; terminal button Molecular Function:aspartate carbamoyltransferase activity; ATP binding; carbamoyl-phosphate synthase (ammonia) activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; dihydroorotase activity; enzyme binding; identical protein binding; protein kinase activity; zinc ion binding Biological Process: 'de novo' pyrimidine base biosynthetic process; arginine biosynthetic process; drug metabolic process; glutamine metabolic process; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; pyrimidine nucleoside biosynthetic process; urea cycle Disease: Congenital Disorder Of Glycosylation, Type Iz
NCBI Summary:	The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
UniProt Code:	P27708
NCBI GenInfo Identifier:	50403731
NCBI Gene ID:	790
NCBI Accession:	P27708.3
UniProt Secondary Accession:	P27708,Q6P0Q0, Q96CK3, D6W552,
UniProt Related Accession:	P27708
Molecular Weight:	242,984 Da
NCBI Full Name:	CAD protein
NCBI Synonym Full Names:	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
NCBI Official Symbol:	CAD
NCBI Official Synonym Symbols:	CDG1Z
NCBI Protein Information:	CAD protein
UniProt Protein Name:	CAD protein
Protein Family:	Cadherin
UniProt Gene Name:	CAD
UniProt Entry Name:	PYR1_HUMAN