Name: Anti-Phospho-ATR-S428 Antibody (CABP0676)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CABP0676
Availability: OutOfStock

Antibody Name:	Anti-Phospho-ATR-S428 Antibody
Antibody SKU:	CABP0676
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NIH/3T3

Immunogen:	A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GISP K
Gene ID:	545
Uniprot:	Q13535
Cellular Location:	Chromosome, Nucleus, PML body
Calculated MW:	294kDa/297kDa/301kDa
Observed MW:	300KDa

Anti-Phospho-ATR-S428 Antibody (CABP0676)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ATR-S428 antibody (CABP0676) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using Phospho-ATR-S428 antibody (CABP0676) at dilution of 1:100. Blue: DAPI for nuclear staining.

Synonyms:

ATR, FCTCS, FRP1, MEC1, SCKL, SCKL1

Background:

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.

UniProt Protein Function:	ATR: a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
UniProt Protein Details:	Protein type:Kinase, lipid; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; Membrane protein, integral; EC 2.7.11.1; ATYPICAL group; PIKK family; ATR subfamily Chromosomal Location of Human Ortholog: 3q23 Cellular Component: XY body; nucleoplasm; Golgi apparatus; PML body; chromosome Molecular Function:protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; regulation of protein binding; multicellular organismal development; protein amino acid autophosphorylation; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA replication; DNA repair; cell cycle; response to DNA damage stimulus; double-strand break repair via homologous recombination Disease: Seckel Syndrome 1; Cutaneous Telangiectasia And Cancer Syndrome, Familial
NCBI Summary:	The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13535
NCBI GenInfo Identifier:	62286460
NCBI Gene ID:	545
NCBI Accession:	Q13535.3
UniProt Secondary Accession:	Q13535,Q59HB2, Q7KYL3, Q93051, Q9BXK4,
UniProt Related Accession:	Q13535
Molecular Weight:	2644
NCBI Full Name:	Serine/threonine-protein kinase ATR
NCBI Synonym Full Names:	ATR serine/threonine kinase
NCBI Official Symbol:	ATR
NCBI Official Synonym Symbols:	FRP1; MEC1; SCKL; FCTCS; SCKL1
NCBI Protein Information:	serine/threonine-protein kinase ATR; FRAP-related protein-1; MEC1, mitosis entry checkpoint 1, homolog; ataxia telangiectasia and Rad3-related protein
UniProt Protein Name:	Serine/threonine-protein kinase ATR
UniProt Synonym Protein Names:	Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1
Protein Family:	Atrophin
UniProt Gene Name:	ATR
UniProt Entry Name:	ATR_HUMAN