Cell Biology Antibodies 16
Anti-Phospho-ARAF-S299 Antibody (CABP0662)
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- SKU:
- CABP0662
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-ARAF-S299 Antibody |
| Antibody SKU: | CABP0662 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S299 of human ARAF (NP_001645.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: |
| Immunogen: | A synthetic phosphorylated peptide around S299 of human ARAF (NP_001645.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RDSG Y |
| Gene ID: | 369 |
| Uniprot: | P10398 |
| Cellular Location: | |
| Calculated MW: | 20kDa/67kDa |
| Observed MW: | Refer to figures |
| Synonyms: | ARAF, A-RAF, ARAF1, PKS2, RAFA1 |
| Background: | This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; TKL group; RAF family Chromosomal Location of Human Ortholog: Xp11.4-p11.2 Cellular Component: cytosol Molecular Function:protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: MAPKKK cascade; negative regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; protein modification process; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway |
| NCBI Summary: | This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012] |
| UniProt Code: | P10398 |
| NCBI GenInfo Identifier: | 1730068 |
| NCBI Gene ID: | 369 |
| NCBI Accession: | P10398.2 |
| UniProt Secondary Accession: | P10398,P07557, Q5H9B2, Q5H9B3, |
| UniProt Related Accession: | P10398 |
| Molecular Weight: | 20,904 Da |
| NCBI Full Name: | Serine/threonine-protein kinase A-Raf |
| NCBI Synonym Full Names: | A-Raf proto-oncogene, serine/threonine kinase |
| NCBI Official Symbol: | ARAF |
| NCBI Official Synonym Symbols: | PKS2; A-RAF; ARAF1; RAFA1 |
| NCBI Protein Information: | serine/threonine-protein kinase A-Raf |
| UniProt Protein Name: | Serine/threonine-protein kinase A-Raf |
| UniProt Synonym Protein Names: | Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks |
| Protein Family: | ABC transporter L-arabinose-binding periplasmic protein |
| UniProt Gene Name: | ARAF |
| UniProt Entry Name: | ARAF_HUMAN |
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