Cell Biology Antibodies 16
Anti-Phospho-AKT1S1-T246 pAb Antibody (CABP0793)
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- SKU:
- CABP0793
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-AKT1S1-T246 Antibody |
| Antibody SKU: | CABP0793 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T246 of human AKT1S1 (NP_001092102.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around T246 of human AKT1S1 (NP_001092102.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LNTS D |
| Gene ID: | 84335 |
| Uniprot: | Q96B36 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 13kDa/27kDa/29kDa |
| Observed MW: | 45kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody (CABP0793) at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| Synonyms: | AKT1S1, Lobe, PRAS40 |
| Background: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
| UniProt Protein Function: | Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
| NCBI Summary: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96B36 |
| NCBI GenInfo Identifier: | 74731194 |
| NCBI Gene ID: | 84335 |
| NCBI Accession: | Q96B36.1 |
| UniProt Secondary Accession: | Q96B36,Q96BI4, Q96IK7, Q96NG2, Q9BWR5, A8MTQ1, B2RE93 J3KPM3, |
| UniProt Related Accession: | Q96B36 |
| Molecular Weight: | 29,262 Da |
| NCBI Full Name: | Proline-rich AKT1 substrate 1 |
| NCBI Synonym Full Names: | AKT1 substrate 1 |
| NCBI Official Symbol: | AKT1S1 |
| NCBI Official Synonym Symbols: | Lobe; PRAS40 |
| NCBI Protein Information: | proline-rich AKT1 substrate 1 |
| UniProt Protein Name: | Proline-rich AKT1 substrate 1 |
| UniProt Synonym Protein Names: | 40 kDa proline-rich AKT substrate |
| Protein Family: | Proline-rich AKT1 substrate |
| UniProt Gene Name: | AKT1S1 |
| UniProt Entry Name: | AKTS1_HUMAN |
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