Cell Death Antibodies 1

Anti-PHLDA3 Antibody (CAB12666)

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SKU:
CAB12666
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-PHLDA3 Antibody
Antibody SKU:CAB12666
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human PHLDA3 (NP_036528.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human
Positive Samples:SKOV3, A-431
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human PHLDA3 (NP_036528.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTAA ATAT VLKE GVLE KRSG GLLQ LWKR KRCV LTER GLQL FEAK GTGG RPKE LSFA RIKA VECV ESTG RHIY FTLV TEGG GEID FRCP LEDP GWNA QITL GLVK FKNQ QAIQ TVRA RQSL GTGT LVS
Gene ID:23612
Uniprot:Q9Y5J5
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:13kDa
Observed MW:16kDa
Synonyms:PHLDA3, TIH1
Background:
UniProt Protein Function:PHLDA3: p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. Belongs to the PHLDA3 family. Induced by p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter.
UniProt Protein Details:

Protein type:Tumor suppressor

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytoplasm; plasma membrane

Molecular Function:phosphatidylinositol 3-phosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-5-phosphate binding

Biological Process: anatomical structure morphogenesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of protein kinase B signaling cascade; positive regulation of apoptosis

UniProt Code:Q9Y5J5
NCBI GenInfo Identifier:74735291
NCBI Gene ID:23612
NCBI Accession:Q9Y5J5.1
UniProt Secondary Accession:Q9Y5J5,Q53HD6, Q8NBW9, B2R5A4,
UniProt Related Accession:Q9Y5J5
Molecular Weight:
NCBI Full Name:Pleckstrin homology-like domain family A member 3
NCBI Synonym Full Names:pleckstrin homology like domain family A member 3
NCBI Official Symbol:PHLDA3  
NCBI Official Synonym Symbols:TIH1  
NCBI Protein Information:pleckstrin homology-like domain family A member 3
UniProt Protein Name:Pleckstrin homology-like domain family A member 3
UniProt Synonym Protein Names:TDAG51/Ipl homolog 1
Protein Family:Pleckstrin homology-like domain family
UniProt Gene Name:PHLDA3  
UniProt Entry Name:PHLA3_HUMAN
Anti-PHLDA3 Antibody (CAB12666)Western blot analysis of extracts of various cell lines, using PHLDA3 antibody (CAB12666) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30min.
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