Name: Anti-PHKG1 Antibody (CAB15701)
Brand: Metabolism Antibodies 1
SKU: CAB15701
Availability: OutOfStock

Antibody Name:	Anti-PHKG1 Antibody
Antibody SKU:	CAB15701
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-350 of human PHKG1 (NP_006204.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-350 of human PHKG1 (NP_006204.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GNYQ FGSP EWDD YSDT VKDL VSRF LVVQ PQNR YTAE EALA HPFF QQYL VEEV RHFS PRGK FKVI ALTV LASV RIYY QYRR VKPV TREI VIRD PYAL RPLR RLID AY
Gene ID:	5260
Uniprot:	Q16816
Cellular Location:
Calculated MW:	45kDa/48kDa
Observed MW:	55kDa

Synonyms:

PHKG1, PHKG

Background:

This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PHKG1: a Ca(2+)/calmodulin-dependent protein kinase of the PHK family. Phosphorylase kinase is a polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin.
UniProt Protein Details:	Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.26; EC 2.7.11.19; CAMK group; PHK family Chromosomal Location of Human Ortholog: 7p11.2 Cellular Component: cytosol Biological Process: glycogen catabolic process
NCBI Summary:	This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q16816
NCBI GenInfo Identifier:	2833281
NCBI Gene ID:	5260
NCBI Accession:	Q16816.3
UniProt Secondary Accession:	Q16816,Q75LP5, B7Z1D0, F5H2S1,
UniProt Related Accession:	Q16816
Molecular Weight:	48,867 Da
NCBI Full Name:	Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
NCBI Synonym Full Names:	phosphorylase kinase catalytic subunit gamma 1
NCBI Official Symbol:	PHKG1
NCBI Official Synonym Symbols:	PHKG
NCBI Protein Information:	phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
UniProt Protein Name:	Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
UniProt Synonym Protein Names:	Phosphorylase kinase subunit gamma-1; Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Protein Family:	Phosphorylase b kinase
UniProt Gene Name:	PHKG1
UniProt Entry Name:	PHKG1_HUMAN

Western blot analysis of extracts of mouse kidney, using PHKG1 antibody (CAB15701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

PHKG1 Antibody (PACO06745)

PHKG1 Antibody (PACO11217)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)