Metabolism Antibodies 1

Anti-PHKA1 Antibody (CAB15700)

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SKU:
CAB15700
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PHKA1 Antibody
Antibody SKU:CAB15700
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Jurkat, HeLa, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QTGK LSEF LTTS CCTH LSFM DPGP EGKL YSED YDDN YDYL ESGN WMND YDST SHAR CGDE VARY LDHL LAHT APHP KLAP TSQK GGLD RFQA AVQT TCDL MSLV TKAK ELHV QNVH MYLP TKLF QASR PSFN LLDS PHPR QENQ VPSV RVEI HLPR DQSG EVDF KALV LQLK ETSS LQEQ ADIL YMLY TMKG PDWN TELY NERS ATVR ELLT ELYG KVGE I
Gene ID:5255
Uniprot:P46020
Cellular Location:Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:129kDa/135kDa/137kDa
Observed MW:137kDa
Synonyms:PHKA1, PHKA
Background:Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.
NCBI Summary:Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]
UniProt Code:P46020
NCBI GenInfo Identifier:169881275
NCBI Gene ID:5255
NCBI Accession:NP_001116142
UniProt Related Accession:P46020
Molecular Weight:129kDa; 135kDa; 137kDa
NCBI Full Name:phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 2
NCBI Synonym Full Names:phosphorylase kinase regulatory subunit alpha 1
NCBI Official Symbol:PHKA1  
NCBI Official Synonym Symbols:PHKA  
NCBI Protein Information:phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
UniProt Protein Name:Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Protein Family:Phosphorylase b kinase regulatory
UniProt Gene Name:PHKA1  
UniProt Entry Name:KPB1_HUMAN
Anti-PHKA1 Antibody (CAB15700)Western blot analysis of extracts of various cell lines, using PHKA1 antibody (CAB15700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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