KO Validated Antibodies 2

Anti-PHGDH Antibody (CAB19974)[KO Validated]

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SKU:
CAB19974
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-PHGDH Antibody [KO Validated]
Antibody SKU:CAB19974
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PHGDH.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human PHGDH.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:26227
Uniprot:O43175
Cellular Location:
Calculated MW:56kDa
Observed MW:57KDa
Anti-PHGDH Antibody [KO Validated] (CAB19974)Western blot analysis of extracts from normal (control) and PHGDH knockout (KO) HeLa cells, using PHGDH antibody (CAB19974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PHGDH, 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA
Background:This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
UniProt Protein Function:PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
UniProt Protein Details:

Protein type:Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol

Molecular Function:electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity

Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process

Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1

NCBI Summary:This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
UniProt Code:O43175
NCBI GenInfo Identifier:21264510
NCBI Gene ID:26227
NCBI Accession:O43175.4
UniProt Secondary Accession:O43175,Q5SZU3, Q9BQ01, B2RD08,
UniProt Related Accession:O43175
Molecular Weight:533
NCBI Full Name:D-3-phosphoglycerate dehydrogenase
NCBI Synonym Full Names:phosphoglycerate dehydrogenase
NCBI Official Symbol:PHGDH  
NCBI Official Synonym Symbols:NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113  
NCBI Protein Information:D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113
UniProt Protein Name:D-3-phosphoglycerate dehydrogenase
UniProt Gene Name:PHGDH  
UniProt Entry Name:SERA_HUMAN
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