Name: Anti-PHF5A Antibody (CAB12678)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12678
Availability: OutOfStock

Antibody Name:	Anti-PHF5A Antibody
Antibody SKU:	CAB12678
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PHF5A (NP_116147.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	Raji, HT-29, HeLa, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PHF5A (NP_116147.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKH HPDL IFCR KQAG VAIG RLCE KCDG KCVI CDSY VRPC TLVR ICDE CNYG SYQG RCVI CGGP GVSD AYYC KECT IQEK DRDG CPKI VNLG SSKT DLFY ERKK YGFK KR
Gene ID:	84844
Uniprot:	Q7RTV0
Cellular Location:	Nucleus, Nucleus speckle
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:

PHF5A, INI, Rds3, SAP14b, SF3B7, SF3b14b, bK223H9.2

Background:

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes.

UniProt Protein Function:	PHF5A: Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing. Belongs to the PHF5 family.
UniProt Protein Details:	Protein type:RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: nucleoplasm; nuclear matrix; nuclear speck; snRNP U2; U12-dependent spliceosome Molecular Function:DNA binding; transcription factor activity Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; positive regulation of transcription, DNA-dependent; gene expression
NCBI Summary:	This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008]
UniProt Code:	Q7RTV0
NCBI GenInfo Identifier:	46577625
NCBI Gene ID:	84844
NCBI Accession:	Q7RTV0.1
UniProt Secondary Accession:	Q7RTV0,Q9UH06,
UniProt Related Accession:	Q7RTV0
Molecular Weight:	Calculated MW: 12kDaObserved MW: 14kDa
NCBI Full Name:	PHD finger-like domain-containing protein 5A
NCBI Synonym Full Names:	PHD finger protein 5A
NCBI Official Symbol:	PHF5A
NCBI Official Synonym Symbols:	INI; Rds3; SF3B7; SAP14b; SF3b14b; bK223H9.2
NCBI Protein Information:	PHD finger-like domain-containing protein 5A; PHD-finger 5a; splicing factor 3b, subunit 7; PHD finger-like domain protein 5A; splicing factor 3B associated 14 kDa protein; splicing factor 3B-associated 14 kDa protein
UniProt Protein Name:	PHD finger-like domain-containing protein 5A
UniProt Synonym Protein Names:	Splicing factor 3B-associated 14 kDa protein; SF3b14b
UniProt Gene Name:	PHF5A
UniProt Entry Name:	PHF5A_HUMAN

Western blot analysis of extracts of various cell lines, using PHF5A antibody (CAB12678) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

PHF5A Antibody (PACO11212)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)