Cell Biology Antibodies 11
Anti-PHF21B Antibody (CAB7849)
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- SKU:
- CAB7849
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PHF21B Antibody |
| Antibody SKU: | CAB7849 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 322-531 of human PHF21B (NP_612424.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse spinal cord, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 322-531 of human PHF21B (NP_612424.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KRLA SNYL NNPL FLTA RANE DPCW KNEI THDE HCAA CKRG ANLQ PCGT CPGA YHLS CLEP PLKT APKG VWVC PRCQ QKAL KKDE GVPW TGML AIVH SYVT HKTV KEEE KQKL LQRG SELQ NEHQ QLEE RDRR LASA VQKC LELK TSLL ARQR GTQS SLDR LRAL LRLI QGEQ LLQV TMTT TSPA PLLA GPWT KPSV AATH PTVQ HPQG HN |
| Gene ID: | 112885 |
| Uniprot: | Q96EK2 |
| Cellular Location: | |
| Calculated MW: | 51kDa/53kDa/57kDa |
| Observed MW: | 50kDa |
| Synonyms: | PHF21B, BHC80L, PHF4 |
| Background: |
| UniProt Protein Function: | PHF21B: 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: nucleus Molecular Function:chromatin binding; histone binding; transcription cofactor activity Biological Process: regulation of transcription, DNA-dependent |
| UniProt Code: | Q96EK2 |
| NCBI GenInfo Identifier: | 15082557 |
| NCBI Gene ID: | 112885 |
| NCBI Accession: | BC012187 |
| UniProt Secondary Accession: | Q96EK2,Q5TFL2, Q6ICC0, B0QYW3, B0QYW4, B3KRU4, B7Z4F8 |
| UniProt Related Accession: | Q96EK2 |
| Molecular Weight: | 51,776 Da |
| NCBI Full Name: | Homo sapiens PHD finger protein 21B, mRNA |
| NCBI Synonym Full Names: | PHD finger protein 21B |
| NCBI Official Symbol: | PHF21B |
| NCBI Official Synonym Symbols: | PHF4; BHC80L |
| NCBI Protein Information: | PHD finger protein 21B |
| UniProt Protein Name: | PHD finger protein 21B |
| Protein Family: | PHD finger protein |
| UniProt Gene Name: | PHF21B |
| UniProt Entry Name: | PF21B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHF21B antibody (CAB7849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human kidney using PHF21B antibody (CAB7849) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using PHF21B antibody (CAB7849) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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