Epigenetics & Nuclear Signaling Antibodies 2
Anti-PHF21A Antibody (CAB6330)
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- SKU:
- CAB6330
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PHF21A Antibody |
| Antibody SKU: | CAB6330 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-680 of human PHF21A (NP_001095272.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, 293T, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-680 of human PHF21A (NP_001095272.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TETT FTFP APVQ PVSL PSPT STDG DIHE DFCS VCRK SGQL LMCD TCSR VYHL DCLD PPLK TIPK GMWI CPRC QDQM LKKE EAIP WPGT LAIV HSYI AYKA AKEE EKQK LLKW SSDL KQER EQLE QKVK QLSN SISK CMEM KNTI LARQ KEMH SSLE KVKQ LIRL IHGI DLSK PVDS EATV GAIS NGPD CTPP ANAA TSTP APSP SSQS CTAN CNQG EETK |
| Gene ID: | 51317 |
| Uniprot: | Q96BD5 |
| Cellular Location: | Nucleus |
| Calculated MW: | 70kDa/74kDa |
| Observed MW: | 70-80kDa |
| Synonyms: | PHF21A, BHC80, BM-006 |
| Background: |
| UniProt Protein Function: | PHF21A: Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: nucleoplasm; histone deacetylase complex Molecular Function:DNA binding; zinc ion binding; chromatin binding Biological Process: establishment and/or maintenance of chromatin architecture; suckling behavior; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; blood coagulation |
| NCBI Summary: | The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q96BD5 |
| NCBI GenInfo Identifier: | 74731224 |
| NCBI Gene ID: | 51317 |
| NCBI Accession: | Q96BD5.1 |
| UniProt Secondary Accession: | Q96BD5,Q6AWA2, Q9C0G7, Q9H8V9, Q9HAK6, Q9NZE9, D3DQP5 |
| UniProt Related Accession: | Q96BD5 |
| Molecular Weight: | 680 |
| NCBI Full Name: | PHD finger protein 21A |
| NCBI Synonym Full Names: | PHD finger protein 21A |
| NCBI Official Symbol: | PHF21A |
| NCBI Official Synonym Symbols: | BHC80; BM-006 |
| NCBI Protein Information: | PHD finger protein 21A; BHC80a; BRAF35/HDAC2 complex (80 kDa); BRAF35-HDAC complex protein BHC80 |
| UniProt Protein Name: | PHD finger protein 21A |
| UniProt Synonym Protein Names: | BHC80a; BRAF35-HDAC complex protein BHC80 |
| Protein Family: | PHD finger protein |
| UniProt Gene Name: | PHF21A |
| UniProt Entry Name: | PF21A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHF21A antibody (CAB6330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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