Epigenetics & Nuclear Signaling Antibodies 4

Anti-PHF19 Antibody (CAB8065)

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SKU:
CAB8065
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:PHF19 Rabbit Polyclonal Antibody
Antibody SKU:CAB8065
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human PHF19 (NP_001009936.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human PHF19 (NP_001009936.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MENR ALDP GTRD SYGA TSHL PNKG ALAK VKNN FKDL MSKL TEGQ YVLC RWTD GLYY LGKI KRVS SSKQ SCLV TFED NSKY WVLW KDIQ HAGV PGEE PKCN ICLG KTSG PLNE ILIC GKCG LGYH QQCH IPIA GSAD QPLL TPWF CRRC IFAL AVRV SLPS SPVP ASPA SSSG ADQR LPSQ SLSS KQKG HTWA LETD SASA TVLG QDL
Gene ID:26147
Uniprot:Q5T6S3
Cellular Location:Nucleus
Calculated MW:14kDa/22kDa/65kDa
Observed MW:66kDa
Synonyms:PHF19, MTF2L1, PCL3, TDRD19B
Background:
UniProt Protein Function:Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases NO66 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
UniProt Code:Q5T6S3
NCBI GenInfo Identifier:74745265
NCBI Gene ID:26147
NCBI Accession:Q5T6S3.1
UniProt Secondary Accession:Q5T6S3,Q32NF2, Q5T6S4, Q6N038, Q8TBL6, Q9UFS9,
UniProt Related Accession:Q5T6S3
Molecular Weight:14,386 Da
NCBI Full Name:PHD finger protein 19
NCBI Synonym Full Names:PHD finger protein 19
NCBI Official Symbol:PHF19  
NCBI Official Synonym Symbols:PCL3; MTF2L1; TDRD19B  
NCBI Protein Information:PHD finger protein 19
UniProt Protein Name:PHD finger protein 19
UniProt Synonym Protein Names:Polycomb-like protein 3; hPCL3
Protein Family:PHD finger protein
UniProt Gene Name:PHF19  
UniProt Entry Name:PHF19_HUMAN
Anti-PHF19 Polyclonal Antibody (CAB8065)Western blot analysis of extracts of various cell lines, using PHF19 antibody (CAB8065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PHF19 Polyclonal Antibody (CAB8065)Immunohistochemistry of paraffin-embedded human oophoroma using PHF19 antibody (CAB8065) at dilution of 1:100 (40x lens).
Anti-PHF19 Polyclonal Antibody (CAB8065)Immunofluorescence analysis of A-549 cells using PHF19 antibody (CAB8065).
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