Epigenetics & Nuclear Signaling Antibodies 4
Anti-PHF1 Antibody (CAB8450)
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- SKU:
- CAB8450
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PHF1 Antibody |
| Antibody SKU: | CAB8450 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PHF1 (NP_077084.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HeLa, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PHF1 (NP_077084.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQP PRLS RSGA SSLW DPAS PAPT SGPR PRLW EGQD VLAR WTDG LLYL GTIK KVDS AREV CLVQ FEDD SQFL VLWK DISP AALP GEEL LCCV CRSE TVVP GNRL VSCE KCRH AYHQ DCHV PRAP APGE GEGT SWVC RQCV FAIA TKRG GALK KGPY ARAM LGMK LSLP YGLK GLDW DAGH LSNR QQSY CYCG GPGE WNLK MLQC RSCL QWFH EACT QCLS KPLL YGDR FYEF ECCV CRGG PEKV RRLQ LRWV DVAH LVLY |
| Gene ID: | 5252 |
| Uniprot: | O43189 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 49kDa/62kDa |
| Observed MW: | 68kDa |
| Synonyms: | PHF1, MTF2L2, PCL1, PHF2, TDRD19C, hPHF1 |
| Background: | This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PHF1: Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cytoplasm; ESC/E(Z) complex; microtubule organizing center; nucleoplasm; nucleus Molecular Function:methylated histone residue binding; protein binding; transcription factor activity; zinc ion binding Biological Process: chromatin modification; gene expression; negative regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to DNA damage stimulus; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | O43189 |
| NCBI GenInfo Identifier: | 317373531 |
| NCBI Gene ID: | 5252 |
| NCBI Accession: | O43189.3 |
| UniProt Secondary Accession: | O43189,O60929, Q5SU07, Q5SU08, Q96KM7, B1AZX2, B1AZX3 |
| UniProt Related Accession: | O43189 |
| Molecular Weight: | 49,643 Da |
| NCBI Full Name: | PHD finger protein 1 |
| NCBI Synonym Full Names: | PHD finger protein 1 |
| NCBI Official Symbol: | PHF1 |
| NCBI Official Synonym Symbols: | PCL1; PHF2; hPHF1; MTF2L2; TDRD19C |
| NCBI Protein Information: | PHD finger protein 1 |
| UniProt Protein Name: | PHD finger protein 1 |
| UniProt Synonym Protein Names: | Polycomb-like protein 1; hPCl1 |
| Protein Family: | PHD finger protein |
| UniProt Gene Name: | PHF1 |
| UniProt Entry Name: | PHF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHF1 antibody (CAB8450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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