Cell Biology Antibodies 5

Anti-PHC2 Antibody (CAB14610)

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SKU:
CAB14610
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PHC2 Antibody
Antibody SKU:CAB14610
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-323 of human PHC2 (NP_004418.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:DU145, HT-29, HeLa, Jurkat, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-323 of human PHC2 (NP_004418.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HFLP SEPT KWNV EDVY EFIR SLPG CQEI AEEF RAQE IDGQ ALLL LKED HLMS AMNI KLGP ALKI YARI SMLK DS
Gene ID:1912
Uniprot:Q8IXK0
Cellular Location:Nucleus
Calculated MW:30kDa/35kDa/87kDa/90kDa
Observed MW:45kDa
Synonyms:PHC2, EDR2, HPH2, PH2
Background:In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PHC2: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: heterochromatin; nucleoplasm; nucleus; PcG protein complex

Molecular Function:DNA binding; identical protein binding; protein binding; zinc ion binding

Biological Process: multicellular organismal development; protein sumoylation; spermatogenesis

NCBI Summary:In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q8IXK0
NCBI GenInfo Identifier:219520710
NCBI Gene ID:1912
NCBI Accession:AAI44122.1
UniProt Secondary Accession:Q8IXK0,Q2TAL3, Q5T0C1, Q6NUJ6, Q6ZQR1, Q8N306, Q8TAG8 Q96BL4, Q9Y4Y7, A1L4Q1, A8KA40, D3DPR2,
UniProt Related Accession:Q8IXK0
Molecular Weight:40kd
NCBI Full Name:PHC2 protein
NCBI Synonym Full Names:polyhomeotic homolog 2
NCBI Official Symbol:PHC2  
NCBI Official Synonym Symbols:PH2; EDR2; HPH2  
NCBI Protein Information:polyhomeotic-like protein 2
UniProt Protein Name:Polyhomeotic-like protein 2
UniProt Synonym Protein Names:Early development regulatory protein 2
Protein Family:Polyhomeotic-like protein
UniProt Gene Name:PHC2  
UniProt Entry Name:PHC2_HUMAN
Anti-PHC2 Antibody (CAB14610)Western blot analysis of extracts of various cell lines, using PHC2 antibody (CAB14610) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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