Antibody Name:	Anti-PHC1 Antibody
Antibody SKU:	CAB5843
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FPVG CSQL LKES EKPL QTGL PTGL TENQ SGGP LGVD SPSA ELDK KANL LKCE YCGK YAPA EQFR GSKR FCSM TCAK RYNV SCSH QFRL KRKK MKEF QEAN YARV RRRG PRRS SSDI ARAK IQGK CHRG QEDS SRGS DNSS YDEA LSPT SPGP LSVR AGHG ERDL GNPN TAPP TPEL HGIN PVFL SSNP SRW
Gene ID:	1911
Uniprot:	P78364
Cellular Location:	Nucleus
Calculated MW:	105kDa
Observed MW:	125kDa/135kDa

Synonyms:

PHC1, EDR1, HPH1, MCPH11, RAE28

Background:

This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein.

UniProt Protein Function:	PHC1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 12p13 Cellular Component: nucleoplasm; nuclear body; sex chromatin; PcG protein complex; nucleus Molecular Function:DNA binding; zinc ion binding; chromatin binding Biological Process: multicellular organismal development; histone ubiquitination Disease: Microcephaly 11, Primary, Autosomal Recessive
NCBI Summary:	This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P78364
NCBI GenInfo Identifier:	224471881
NCBI Gene ID:	1911
NCBI Accession:	P78364.3
UniProt Secondary Accession:	P78364,Q8WVM3, Q9BU63, D3DUV4,
UniProt Related Accession:	P78364
Molecular Weight:	Calculated MW: 105kDaObserved MW: 125kDa/135kDa
NCBI Full Name:	Polyhomeotic-like protein 1
NCBI Synonym Full Names:	polyhomeotic homolog 1 (Drosophila)
NCBI Official Symbol:	PHC1
NCBI Official Synonym Symbols:	EDR1; HPH1; RAE28; MCPH11
NCBI Protein Information:	polyhomeotic-like protein 1; polyhomeotic-like 1; early development regulatory protein 1; early development regulator 1 (homolog of polyhomeotic 1)
UniProt Protein Name:	Polyhomeotic-like protein 1
UniProt Synonym Protein Names:	Early development regulatory protein 1
Protein Family:	Polyhomeotic-like protein
UniProt Gene Name:	PHC1
UniProt Entry Name:	PHC1_HUMAN

	Western blot analysis of extracts of various cell lines, using PHC1 Antibody (CAB5843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human adenomyosis using PHC1 antibody (CAB5843) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using PHC1 antibody (CAB5843) at dilution of 1:100 (40x lens).