Name: Anti-PHB2 Antibody (CAB4504)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB4504
Availability: OutOfStock

Antibody Name:	Anti-PHB2 Antibody
Antibody SKU:	CAB4504
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human PHB2 (NP_001138303.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, 22Rv1, 293T, HepG2, A-549, Mouse heart, Mouse liver, Rat heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human PHB2 (NP_001138303.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQN LKDL AGRL PAGP RGMG TALK LLLG AGAV AYGV RESV FTVE GGHR AIFF NRIG GVQQ DTIL AEGL HFRI PWFQ YPII YDIR ARPR KISS PTGS KDLQ MVNI SLRV LSRP NAQE LPSM YQRL GLDY EERV LPSI VNEV LKSV VAKF NASQ LITQ RAQV SLLI RREL TERA KDFS LILD DVAI TELS FSRE YTAA VEAK QVAQ QEAQ RAQF LVEK AKQE QRQK IVQA EGEA EAAK MLGE ALSK NPGY IKLR KIRA AQNI SKTI ATSQ NRIY LTAD NLVL NLQD ESFT RGSD SLIK GKK
Gene ID:	11331
Uniprot:	Q99623
Cellular Location:	Cytoplasm, Mitochondrion inner membrane, Nucleus
Calculated MW:	29kDa/33kDa
Observed MW:	32kDa

Synonyms:	PHB2, BAP, BCAP37, Bap37, PNAS-141, REA, hBAP, p22
Background:

UniProt Protein Function:	BAP37: a protein of the band 7 family. This family includes the prohibitins, cytoplasmic anti-proliferative proteins and stomatin, an erythrocyte membrane protein. A repressor of estrogen receptor (ER) activity. Reverses steroid receptor coactivator 1 enhancement of ER activity. May play a role in determining the sensitivity of estrogen target cells to antiestrogen and estrogen therapy.
UniProt Protein Details:	Protein type:Mitochondrial; Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 12p13 Cellular Component: protein complex; nuclear matrix; mitochondrion; mitochondrial inner membrane; nucleus Molecular Function:protein binding; estrogen receptor binding Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; negative regulation of mammary gland epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway
UniProt Code:	Q99623
NCBI GenInfo Identifier:	74752151
NCBI Gene ID:
NCBI Accession:	Q99623.2
Molecular Weight:
NCBI Full Name:	Prohibitin-2
UniProt Protein Name:	Prohibitin-2
UniProt Synonym Protein Names:	B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity
Protein Family:	Readthrough protein
UniProt Gene Name:	PHB2
UniProt Entry Name:	PHB2_HUMAN

	Western blot analysis of extracts of various cell lines, using PHB2 antibody (CAB4504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human esophagus using PHB2 antibody (CAB4504) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using PHB2 Polyclonal Antibody (CAB4504) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PHB2 Polyclonal Antibody (CAB4504) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.