Anti-PGP9.5/UCHL1 Antibody (CAB20380)
- SKU:
- CAB20380
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-PGP9.5/UCHL1 Antibody (CAB20380) |
| Antibody SKU: | CAB20380 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PGP95/UCHL1 (NP_004172.2). |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, DU145, SH-SY5Y, A-549, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PGP95/UCHL1 (NP_004172.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQLK PMEI NPEM LNKV LSRL GVAG QWRF VDVL GLEE ESLG SVPA PACA LLLL FPLT AQHE NFRK KQIE ELKG QEVS PKVY FMKQ TIGN SCGT IGLI HAVA NNQD KLGF EDGS VLKQ FLSE TEKM SPED RAKC FEKN EAIQ AAHD AVAQ EGQC RVDD KVNF HFIL FNNV DGHL YELD GRMP FPVN HGAS SEDT LLKD AAKV CREF TERE QGEV RFSA VALC |
| Cellular Location: | |
| Calculated MW: | 25kDa |
| Observed MW: | 27KDa |
| Synonyms: | HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1, UCHL1 |
| Background: | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.provided by RefSeq, Sep 2009 |
 | Immunofluorescence analysis of SH-SY5Y cells using PGP9. 5/UCHL1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using PGP9. 5/UCHL1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded human brain using PGP9. 5/PGP9. 5/UCHL1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of A-549 cells using PGP9. 5/UCHL1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using UCHL1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
Related Products
PGP9.5/UCHL1 Monoclonal Antibody (CAB22415)
PGP9.5/UCHL1 Monoclonal Antibody (CAB22415)The product page for this antibody is being prepared. If you have a question please don't hesitate to get in touch!
Human UCHL1/PGP9.5 Recombinant Protein (RPES1003)
system_update_altDatasheetHuman UCHL1/PGP9.5 Recombinant Protein Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqut
Rat UCHL1/PGP9.5 Recombinant Protein (RPES1018)
Signal Transduction Recombinant Proteins
system_update_altDatasheetRat UCHL1/PGP9.5 Recombinant Protein Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutin
Mouse UCHL1/PGP9.5 Recombinant Protein (RPES0216)
Signal Transduction Recombinant Proteins
system_update_altDatasheetMouse UCHL1/PGP9.5 Recombinant Protein Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqut