Cell Biology Antibodies 12
Anti-PGLS Antibody (CAB8366)
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- SKU:
- CAB8366
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PGLS Antibody |
| Antibody SKU: | CAB8366 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human PGLS (NP_036220.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, THP-1, HepG2, HeLa, U-251MG, Mouse heart, Mouse spleen, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human PGLS (NP_036220.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAP APGL ISVF SSSQ ELGA ALAQ LVAQ RAAC CLAG ARAR FALG LSGG SLVS MLAR ELPA AVAP AGPA SLAR WTLG FCDE RLVP FDHA ESTY GLYR THLL SRLP IPES QVIT INPE LPVE EAAE DYAK KLRQ AFQG DSIP VFDL LILG VGPD GHTC SLFP DHPL LQER EKIV APIS DSPK PPPQ RVTL TLPV LNAA RTVI FVAT GEGK AAVL KRIL EDQE ENPL PAAL VQPH TGKL CWFL DEAA ARLL TVPF EKHS TL |
| Gene ID: | 25796 |
| Uniprot: | O95336 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 27kDa |
| Observed MW: | 28kDa |
| Synonyms: | PGLS, 6PGL, HEL-S-304 |
| Background: |
| UniProt Protein Function: | PGLS: Hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily. |
| UniProt Protein Details: | Protein type:EC 3.1.1.31; Hydrolase; Carbohydrate Metabolism - pentose phosphate pathway Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: cytosol Molecular Function:6-phosphogluconolactonase activity; protein binding Biological Process: pentose-phosphate shunt |
| UniProt Code: | O95336 |
| NCBI GenInfo Identifier: | 7387511 |
| NCBI Gene ID: | 25796 |
| NCBI Accession: | O95336.2 |
| UniProt Related Accession: | O95336 |
| Molecular Weight: | 27,547 Da |
| NCBI Full Name: | 6-phosphogluconolactonase |
| NCBI Synonym Full Names: | 6-phosphogluconolactonase |
| NCBI Official Symbol: | PGLS |
| NCBI Official Synonym Symbols: | 6PGL; HEL-S-304 |
| NCBI Protein Information: | 6-phosphogluconolactonase |
| UniProt Protein Name: | 6-phosphogluconolactonase |
| UniProt Gene Name: | PGLS |
| UniProt Entry Name: | 6PGL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PGLS antibody (CAB8366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat stomach using PGLS Rabbit pAb (CAB8366) at dilution of 1:200 (40x lens). |
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