Metabolism Antibodies 1
Anti-PGK2 Antibody (CAB12952)
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- SKU:
- CAB12952
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PGK2 Antibody |
| Antibody SKU: | CAB12952 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Jurkat, HeLa, HepG2, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YTFL KVLN NMEI GASL FDEE GAKI VKDI MAKA QKNG VRIT FPVD FVTG DKFD ENAQ VGKA TVAS GISP GWMG LDCG PESN KNHA QVVA QARL IVWN GPLG VFEW DAFA KGTK ALMD EIV |
| Gene ID: | 5232 |
| Uniprot: | P07205 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 44kDa |
| Observed MW: | 45kDa |
| Synonyms: | PGK2, HEL-S-272, PGKB, PGKPS, dJ417L20.2 |
| Background: | This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. |
| UniProt Protein Function: | PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3 Chromosomal Location of Human Ortholog: 6p12.3 Cellular Component: nucleus Molecular Function:ATP binding; phosphoglycerate kinase activity Biological Process: phosphorylation |
| NCBI Summary: | This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010] |
| UniProt Code: | P07205 |
| NCBI GenInfo Identifier: | 21264485 |
| NCBI Gene ID: | 5232 |
| NCBI Accession: | P07205.3 |
| UniProt Secondary Accession: | P07205,Q9H107, B2R6Y8, |
| UniProt Related Accession: | P07205 |
| Molecular Weight: | 44,796 Da |
| NCBI Full Name: | Phosphoglycerate kinase 2 |
| NCBI Synonym Full Names: | phosphoglycerate kinase 2 |
| NCBI Official Symbol: | PGK2 |
| NCBI Official Synonym Symbols: | PGKB; PGKPS; HEL-S-272; dJ417L20.2 |
| NCBI Protein Information: | phosphoglycerate kinase 2 |
| UniProt Protein Name: | Phosphoglycerate kinase 2 |
| UniProt Synonym Protein Names: | Phosphoglycerate kinase, testis specific |
| Protein Family: | Phosphoglycerate kinase |
| UniProt Gene Name: | PGK2 |
| UniProt Entry Name: | PGK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PGK2 antibody (CAB12952) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (CAB12952) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (CAB12952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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