Metabolism Antibodies 1

Anti-PGK1 Antibody (CAB14039)

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SKU:
CAB14039
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PGK1 Antibody
Antibody SKU:CAB14039
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, U-251MG, Mouse brain, Mouse liver, Mouse testis, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSLS NKLT LDKL DVKG KRVV MRVD FNVP MKNN QITN NQRI KAAV PSIK FCLD NGAK SVVL MSHL GRPD GVPM PDKY SLEP VAVE LKSL LGKD VLFL KDCV GPEV EKAC ANPA AGSV ILLE NLRF HVEE EGKG KDAS GNKV KAEP AKIE AFRA SLSK LGDV YVND AFGT AHRA HSSM VGVN LPQK AGGF LMKK ELNY FAKA LESP ERPF LAIL GGAK VADK IQLI NNML DKVN EMII GGGM AFTF LKVL NNME IGTS LFDE EGAK IVKD LMSK AEKN GVKI TLPV DFVT ADKF DENA KTGQ ATVA SGIP AGWM GLDC GPES SKKY AEAV TRAK QIVW NGPV GVFE WEAF ARGT KALM DEVV KATS RGCI TIIG GGDT ATCC AKWN TEDK VSHV STGG GASL ELLE GKVL PGVD ALSN I
Gene ID:5230
Uniprot:P00558
Cellular Location:Cytoplasm
Calculated MW:41kDa/44kDa
Observed MW:44kDa
Synonyms:PGK1, HEL-S-68p, MIG10, PGKA
Background:The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
UniProt Protein Function:PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein).
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3

Chromosomal Location of Human Ortholog: Xq13.3

Cellular Component: membrane; cytosol

Molecular Function:protein binding; phosphoglycerate kinase activity; ATP binding

Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis

Disease: Phosphoglycerate Kinase 1 Deficiency

NCBI Summary:The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]
UniProt Code:P00558
NCBI GenInfo Identifier:52788229
NCBI Gene ID:5230
NCBI Accession:P00558.3
UniProt Secondary Accession:P00558,Q5J7W1, Q6IBT6, Q8NI87, A8K4W6, B7Z7A9,
UniProt Related Accession:P00558
Molecular Weight:417
NCBI Full Name:Phosphoglycerate kinase 1
NCBI Synonym Full Names:phosphoglycerate kinase 1
NCBI Official Symbol:PGK1  
NCBI Official Synonym Symbols:PGKA; MIG10; HEL-S-68p  
NCBI Protein Information:phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p
UniProt Protein Name:Phosphoglycerate kinase 1
UniProt Synonym Protein Names:Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2
Protein Family:Phosphoglycerate kinase
UniProt Gene Name:PGK1  
UniProt Entry Name:PGK1_HUMAN
Anti-PGK1 Antibody (CAB14039)Western blot analysis of extracts of various cell lines, using PGK1 antibody (CAB14039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-PGK1 Antibody (CAB14039)Immunohistochemistry of paraffin-embedded rat intestine using PGK1 Antibody (CAB14039) at dilution of 1:100 (40x lens).
Anti-PGK1 Antibody (CAB14039)Immunohistochemistry of paraffin-embedded human colon using PGK1 Antibody (CAB14039) at dilution of 1:100 (40x lens).
Anti-PGK1 Antibody (CAB14039)Immunohistochemistry of paraffin-embedded human esophagus using PGK1 Antibody (CAB14039) at dilution of 1:100 (40x lens).
Anti-PGK1 Antibody (CAB14039)Immunohistochemistry of paraffin-embedded mouse kidney using PGK1 Antibody (CAB14039) at dilution of 1:100 (40x lens).
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