Metabolism Antibodies 2

Anti-PGAM2 Antibody (CAB7917)

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SKU:
CAB7917
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PGAM2 Antibody
Antibody SKU:CAB7917
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human PGAM2 (NP_000281.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:22Rv1, SH-SY5Y, U-87MG, Mouse kidney, Mouse testis, Mouse skeletal muscle, Rat brain, Rat kidney, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human PGAM2 (NP_000281.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATH RLVM VRHG ESTW NQEN RFCG WFDA ELSE KGTE EAKR GAKA IKDA KMEF DICY TSVL KRAI RTLW AILD GTDQ MWLP VVRT WRLN ERHY GGLT GLNK AETA AKHG EEQV KIWR RSFD IPPP PMDE KHPY YNSI SKER RYAG LKPG ELPT CESL KDTI ARAL PFWN EEIV PQIK AGKR VLIA AHGN SLRG IVKH LEGM SDQA IMEL NLPT GIPI VYEL NKEL KPTK PMQF LGDE ETVR KAME AVAA QGKA K
Gene ID:5224
Uniprot:P15259
Cellular Location:
Calculated MW:28kDa
Observed MW:29kDa
Synonyms:PGAM2, GSD10, PGAM-M, PGAMM
Background:Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.
UniProt Code:P15259
NCBI GenInfo Identifier:50593010
NCBI Gene ID:5224
NCBI Accession:NP_000281.2
UniProt Related Accession:P15259
Molecular Weight:28,766 Da
NCBI Full Name:phosphoglycerate mutase 2
NCBI Synonym Full Names:phosphoglycerate mutase 2
NCBI Official Symbol:PGAM2  
NCBI Official Synonym Symbols:GSD10; PGAMM; PGAM-M  
NCBI Protein Information:phosphoglycerate mutase 2
UniProt Protein Name:Phosphoglycerate mutase 2
UniProt Synonym Protein Names:BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM-M
Protein Family:Phosphoglycerate mutase
UniProt Gene Name:PGAM2  
Anti-PGAM2 Antibody (CAB7917)Western blot analysis of extracts of various cell lines, using PGAM2 antibody (CAB7917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-PGAM2 Antibody (CAB7917)Immunofluorescence analysis of rat heart using PGAM2 Rabbit pAb (CAB7917) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-PGAM2 Antibody (CAB7917)Immunofluorescence analysis of mouse heart using PGAM2 Rabbit pAb (CAB7917) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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