Metabolism Antibodies 2

Anti-PGAM1 Antibody (CAB4015)

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SKU:
CAB4015
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-PGAM1 Antibody
Antibody SKU:CAB4015
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, LO2, A375, MCF7, NIH/3T3, Mouse brain, Mouse kidney, Mouse liver, Rat spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAY KLVL IRHG ESAW NLEN RFSG WYDA DLSP AGHE EAKR GGQA LRDA GYEF DICF TSVQ KRAI RTLW TVLD AIDQ MWLP VVRT WRLN ERHY GGLT GLNK AETA AKHG EAQV KIWR RSYD VPPP PMEP DHPF YSNI SKDR RYAD LTED QLPS CESL KDTI ARAL PFWN EEIV PQIK EGKR VLIA AHGN SLRG IVKH LEGL SEEA IMEL NLPT GIPI VYEL DKNL KPIK PMQF LGDE ETVR KAME AVAA QGKA KK
Gene ID:5223
Uniprot:P18669
Cellular Location:
Calculated MW:28kDa
Observed MW:29kDa
Synonyms:PGAM1, HEL-S-35, PGAM-B, PGAMA
Background:The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
UniProt Protein Details:

Protein type:EC 5.4.2.4; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.11

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding

Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis

NCBI Summary:Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]
UniProt Code:P18669
NCBI GenInfo Identifier:130348
NCBI Gene ID:5223
NCBI Accession:P18669.2
UniProt Secondary Accession:P18669,Q9BWC0,
UniProt Related Accession:P18669
Molecular Weight:254
NCBI Full Name:Phosphoglycerate mutase 1
NCBI Synonym Full Names:phosphoglycerate mutase 1 (brain)
NCBI Official Symbol:PGAM1  
NCBI Official Synonym Symbols:PGAMA; PGAM-B; HEL-S-35  
NCBI Protein Information:phosphoglycerate mutase 1; BPG-dependent PGAM 1; phosphoglycerate mutase isozyme B; epididymis secretory protein Li 35; phosphoglycerate mutase A, nonmuscle form
UniProt Protein Name:Phosphoglycerate mutase 1
UniProt Synonym Protein Names:BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B
Protein Family:Phosphoglycerate mutase
UniProt Gene Name:PGAM1  
UniProt Entry Name:PGAM1_HUMAN
Anti-PGAM1 Antibody (CAB4015)Western blot analysis of extracts of various cell lines, using PGAM1 antibody (CAB4015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-PGAM1 Antibody (CAB4015)Immunofluorescence analysis of L929 cells using PGAM1 Rabbit pAb (CAB4015) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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