Cell Biology Antibodies 8
Anti-PFN2 Antibody (CAB3074)
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- SKU:
- CAB3074
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PFN2 Antibody |
| Antibody SKU: | CAB3074 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human PFN2 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, BT-474, U-251MG, Mouse skeletal muscle, Mouse kidney, Rat brain |
| Immunogen: | A synthetic peptide of human PFN2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5217 |
| Uniprot: | P35080 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 15kDa |
| Observed MW: | 15kDa |
| Synonyms: | PFN2, D3S1319E, PFL |
| Background: | The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. |
| UniProt Protein Function: | profilin 2: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Belongs to the profilin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 3q25.1 Cellular Component: cytoskeleton; cytoplasm; terminal button Molecular Function:actin monomer binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; adenyl-nucleotide exchange factor activity Biological Process: positive regulation of actin filament polymerization; positive regulation of ATPase activity; negative regulation of actin filament polymerization; positive regulation of stress fiber formation; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation |
| NCBI Summary: | The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35080 |
| NCBI GenInfo Identifier: | 20178322 |
| NCBI Gene ID: | 5217 |
| NCBI Accession: | P35080.3 |
| UniProt Secondary Accession: | P35080,Q4VBQ4, Q8WVF9, Q9HBK2, B2R4C8, D3DNI4, |
| UniProt Related Accession: | P35080 |
| Molecular Weight: | 140 |
| NCBI Full Name: | Profilin-2 |
| NCBI Synonym Full Names: | profilin 2 |
| NCBI Official Symbol: | PFN2 |
| NCBI Official Synonym Symbols: | PFL; D3S1319E |
| NCBI Protein Information: | profilin-2; profilin II |
| UniProt Protein Name: | Profilin-2 |
| UniProt Synonym Protein Names: | Profilin II |
| UniProt Gene Name: | PFN2 |
| UniProt Entry Name: | PROF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFN2 antibody (CAB3074) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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