KO Validated Antibodies 1

Anti-PFKP Antibody (CAB7916)[KO Validated]

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SKU:
CAB7916
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PFKP Antibody [KO Validated]
Antibody SKU:CAB7916
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PFKP
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, SKOV3, HeLa, NCI-H460, Mouse brain, Rat heart
Immunogen:Recombinant protein of human PFKP
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5214
Uniprot:Q01813
Cellular Location:Cytoplasm
Calculated MW:85kDa
Observed MW:100kDa
Synonyms:PFKP, ATP-PFK, PFK-C, PFK-P, PFKF
Background:This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: cytosol; membrane; nucleus

Molecular Function:6-phosphofructokinase activity; protein complex binding

NCBI Summary:The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]
UniProt Code:Q01813
NCBI GenInfo Identifier:1346355
NCBI Gene ID:5214
NCBI Accession:Q01813.2
UniProt Secondary Accession:Q01813,Q5VSR7, Q5VSR8, B3KS15,
UniProt Related Accession:Q01813
Molecular Weight:85596 Da
NCBI Full Name:ATP-dependent 6-phosphofructokinase, platelet type
NCBI Synonym Full Names:phosphofructokinase, platelet
NCBI Official Symbol:PFKP  
NCBI Official Synonym Symbols:PFKF; PFK-C; PFK-P; ATP-PFK  
NCBI Protein Information:ATP-dependent 6-phosphofructokinase, platelet type
UniProt Protein Name:ATP-dependent 6-phosphofructokinase, platelet type
UniProt Synonym Protein Names:6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase
Protein Family:ATP-dependent 6-phosphofructokinase
UniProt Gene Name:PFKP  
UniProt Entry Name:PFKAP_HUMAN
Anti-PFKP Antibody [KO Validated] (CAB7916)Western blot analysis of extracts of various cell lines, using PFKP antibody (CAB7916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-PFKP Antibody [KO Validated] (CAB7916)Western blot analysis of extracts from normal (control) and PFKP knockout (KO) HeLa cells, using PFKP antibody (CAB7916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-PFKP Antibody [KO Validated] (CAB7916)Immunohistochemistry of paraffin-embedded rat brain using PFKP antibody (CAB7916) (40x lens).
Anti-PFKP Antibody [KO Validated] (CAB7916)Immunohistochemistry of paraffin-embedded mouse brain using PFKP antibody (CAB7916) (40x lens).
Anti-PFKP Antibody [KO Validated] (CAB7916)Immunofluorescence analysis of U-2 OS cells using PFKP antibody (CAB7916) at dilution of 1:100. Blue: DAPI for nuclear staining.
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