Metabolism Antibodies 3

Anti-PFKM Antibody (CAB3671)

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SKU:
CAB3671
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-PFKM Antibody
Antibody SKU:CAB3671
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, RD, Mouse skeletal muscle, Mouse brain, Rat skeletal muscle
Immunogen:A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5213
Uniprot:P08237
Cellular Location:
Calculated MW:81kDa
Observed MW:90KDa
Synonyms:ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122
Background:Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
UniProt Protein Function:PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Kinase, other

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol

Molecular Function:6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein homodimerization activity

Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; fructose 6-phosphate metabolic process; glucose homeostasis; glucose metabolic process; glycogen catabolic process; glycolysis; muscle maintenance; positive regulation of insulin secretion; protein oligomerization

Disease: Glycogen Storage Disease Vii

NCBI Summary:Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
UniProt Code:P08237
NCBI GenInfo Identifier:125126
NCBI Gene ID:5213
NCBI Accession:P08237.2
UniProt Secondary Accession:P08237,Q16814, Q16815, Q6ZTT1, J3KNX3,
UniProt Related Accession:P08237
Molecular Weight:93,254 Da
NCBI Full Name:ATP-dependent 6-phosphofructokinase, muscle type
NCBI Synonym Full Names:phosphofructokinase, muscle
NCBI Official Symbol:PFKM  
NCBI Official Synonym Symbols:GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122  
NCBI Protein Information:ATP-dependent 6-phosphofructokinase, muscle type
UniProt Protein Name:ATP-dependent 6-phosphofructokinase, muscle type
UniProt Synonym Protein Names:6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase
Protein Family:ATP-dependent 6-phosphofructokinase
UniProt Gene Name:PFKM  
UniProt Entry Name:PFKAM_HUMAN
Anti-PFKM Antibody (CAB3671)Western blot - PFKM Rabbit mAb (CAB3671)
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