Metabolism Antibodies 2
Anti-PFKL Antibody (CAB7708)
- SKU:
- CAB7708
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PFKL Antibody |
| Antibody SKU: | CAB7708 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FANA PDSA CVIG LKKK AVAF SPVT ELKK DTDF EHRM PREQ WWLS LRLM LKML AQYR ISMA AYVS GELE HVTR RTLS MDKG F |
| Gene ID: | 5211 |
| Uniprot: | P17858 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 85kDa/90kDa |
| Observed MW: | 85kDa |
| Synonyms: | PFKL, ATP-PFK, PFK-B, PFK-L |
| Background: | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1, 6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Kinase, other; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: 6-phosphofructokinase complex; cytosol; membrane Molecular Function:6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolysis; protein oligomerization; response to glucose stimulus |
| NCBI Summary: | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
| UniProt Code: | P17858 |
| NCBI GenInfo Identifier: | 134048493 |
| NCBI Gene ID: | 5211 |
| NCBI Accession: | P17858.6 |
| UniProt Secondary Accession: | P17858,Q96A64, Q96IH4, Q9BR91, |
| UniProt Related Accession: | P17858 |
| Molecular Weight: | 90,203 Da |
| NCBI Full Name: | ATP-dependent 6-phosphofructokinase, liver type |
| NCBI Synonym Full Names: | phosphofructokinase, liver type |
| NCBI Official Symbol: | PFKL |
| NCBI Official Synonym Symbols: | PFK-B; PFK-L; ATP-PFK |
| NCBI Protein Information: | ATP-dependent 6-phosphofructokinase, liver type |
| UniProt Protein Name: | ATP-dependent 6-phosphofructokinase, liver type |
| UniProt Synonym Protein Names: | 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase |
| Protein Family: | ATP-dependent 6-phosphofructokinase |
| UniProt Gene Name: | PFKL |
| UniProt Entry Name: | PFKAL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFKL antibody (CAB7708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PFKL antibody (CAB7708) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using PFKL antibody (CAB7708) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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