Metabolism Antibodies 1

Anti-PFKL Antibody (CAB15080)

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SKU:
CAB15080
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PFKL Antibody
Antibody SKU:CAB15080
Antibody Size:20uL, 50uL, 100uL
Application:IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 601-780 of human PFKL (NP_002617.3).
Application:IF
Recommended Dilution:IF 1:50 - 1:200
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 601-780 of human PFKL (NP_002617.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IHDL KVNV EHMT EKMK TDIQ RGLV LRNE KCHD YYTT EFLY NLYS SEGK GVFD CRTN VLGH LQQG GAPT PFDR NYGT KLGV KAML WLSE KLRE VYRK GRVF ANAP DSAC VIGL KKKA VAFS PVTE LKKD TDFE HRMP REQW WLSL RLML KMLA QYRI SMAA YVSG ELEH VTRR TLSM DKGF
Gene ID:5211
Uniprot:P17858
Cellular Location:Cytoplasm
Calculated MW:85kDa/90kDa
Observed MW:
Synonyms:PFKL, ATP-PFK, PFK-B, PFK-L
Background:This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1, 6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Kinase, other; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; cytosol; membrane

Molecular Function:6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolysis; protein oligomerization; response to glucose stimulus

NCBI Summary:This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Code:P17858
NCBI GenInfo Identifier:134048493
NCBI Gene ID:5211
NCBI Accession:P17858.6
UniProt Secondary Accession:P17858,Q96A64, Q96IH4, Q9BR91,
UniProt Related Accession:P17858
Molecular Weight:90,203 Da
NCBI Full Name:ATP-dependent 6-phosphofructokinase, liver type
NCBI Synonym Full Names:phosphofructokinase, liver type
NCBI Official Symbol:PFKL  
NCBI Official Synonym Symbols:PFK-B; PFK-L; ATP-PFK  
NCBI Protein Information:ATP-dependent 6-phosphofructokinase, liver type
UniProt Protein Name:ATP-dependent 6-phosphofructokinase, liver type
UniProt Synonym Protein Names:6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase
Protein Family:ATP-dependent 6-phosphofructokinase
UniProt Gene Name:PFKL  
UniProt Entry Name:PFKAL_HUMAN
Anti-PFKL Antibody (CAB15080)Immunofluorescence analysis of U2OS cells using PFKL antibody (CAB15080) at dilution of 1:100. Blue: DAPI for nuclear staining.
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