Cell Biology Antibodies 12

Anti-PFKFB2 Antibody (CAB9311)

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SKU:
CAB9311
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PFKFB2 Antibody
Antibody SKU:CAB9311
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Mouse liver, Mouse brain, Mouse skeletal muscle, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RDKP TNNF PKNQ TPVR MRRN SFTP LSSS NTIR RPRN YSVG SRPL KPLS PLRA QDMQ EGAD
Gene ID:5208
Uniprot:O60825
Cellular Location:
Calculated MW:54kDa/58kDa
Observed MW:54kDa
Synonyms:PFKFB2, PFK-2/FBPase-2
Background:The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate, and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of fructose-2, 6-bisphosphate. This protein regulates fructose-2, 6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2, 6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:EC 3.1.3.46; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; Kinase, other; Phosphatase (non-protein); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: cytosol

Molecular Function:6-phosphofructo-2-kinase activity; protein binding; protein kinase binding

Biological Process: glycolysis

NCBI Summary:The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O60825
NCBI GenInfo Identifier:12643333
NCBI Gene ID:5208
NCBI Accession:O60825.2
UniProt Secondary Accession:O60825,O60824, Q5VVQ3, Q5VVQ4, Q9H3P1,
UniProt Related Accession:O60825
Molecular Weight:54,406 Da
NCBI Full Name:6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
NCBI Synonym Full Names:6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
NCBI Official Symbol:PFKFB2  
NCBI Official Synonym Symbols:PFK-2/FBPase-2  
NCBI Protein Information:6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
UniProt Protein Name:6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
UniProt Synonym Protein Names:6PF-2-K/Fru-2,6-P2ase heart-type isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Protein Family:6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
UniProt Gene Name:PFKFB2  
UniProt Entry Name:F262_HUMAN
Anti-PFKFB2 Polyclonal Antibody (CAB9311)Western blot analysis of extracts of various cell lines, using PFKFB2 antibody (CAB9311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-PFKFB2 Polyclonal Antibody (CAB9311)Immunofluorescence analysis of C6 cells using PFKFB2 Rabbit pAb (CAB9311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-PFKFB2 Polyclonal Antibody (CAB9311)Immunofluorescence analysis of L929 cells using PFKFB2 Rabbit pAb (CAB9311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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