Cell Biology Antibodies 5
Anti-PFDN4 Antibody (CAB15300)
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- SKU:
- CAB15300
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PFDN4 Antibody |
| Antibody SKU: | CAB15300 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human PFDN4 (NP_002614.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | DU145, HeLa, 293T, MCF7, mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human PFDN4 (NP_002614.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAT MKKA AAED VNVT FEDQ QKIN KFAR NTSR ITEL KEEI EVKK KQLQ NLED ACDD IMLA DDDC LMIP YQIG DVFI SHSQ EETQ EMLE EAKK NLQE EIDA LESR VESI QRVL ADLK VQLY AKFG SNIN LEAD ES |
| Gene ID: | 5203 |
| Uniprot: | Q9NQP4 |
| Cellular Location: | Cytoplasm, Mitochondrion, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
| Synonyms: | PFDN4, C1, PFD4 |
| Background: | This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. |
| UniProt Protein Function: | PFDN4: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol; prefoldin complex Molecular Function:protein binding; chaperone binding; unfolded protein binding Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding |
| NCBI Summary: | This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQP4 |
| NCBI GenInfo Identifier: | 12643815 |
| NCBI Gene ID: | 5203 |
| NCBI Accession: | Q9NQP4.1 |
| UniProt Secondary Accession: | Q9NQP4,Q5TD11, Q92779, |
| UniProt Related Accession: | Q9NQP4 |
| Molecular Weight: | Calculated: 15kDaObserved: 15kDa |
| NCBI Full Name: | Prefoldin subunit 4 |
| NCBI Synonym Full Names: | prefoldin subunit 4 |
| NCBI Official Symbol: | PFDN4 |
| NCBI Official Synonym Symbols: | C1; PFD4 |
| NCBI Protein Information: | prefoldin subunit 4; prefoldin 4; protein C-1 |
| UniProt Protein Name: | Prefoldin subunit 4 |
| UniProt Synonym Protein Names: | Protein C-1 |
| Protein Family: | Prefoldin |
| UniProt Gene Name: | PFDN4 |
| UniProt Entry Name: | PFD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFDN4 antibody (CAB15300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using PFDN4 antibody (CAB15300) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using PFDN4 antibody (CAB15300) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using PFDN4 antibody (CAB15300) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using PFDN4 antibody (CAB15300) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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