Name: Anti-PEX16 Antibody (CAB10387)
Brand: Metabolism Antibodies 1
SKU: CAB10387
Availability: OutOfStock

Antibody Name:	Anti-PEX16 Antibody
Antibody SKU:	CAB10387
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 132-336 of human PEX16 (NP_004804.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, MCF7, HT-1080, SW620, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 132-336 of human PEX16 (NP_004804.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KAGL QTSP PIVP LDRE TQAQ PPDG DHSP GNHE QSYV GKRS NRVV RTLQ NTPS LHSR HWGA PQQR EGRQ QQHH EELS ATPT PLGL QETI AEFL YIAR PLLH LLSL GLWG QRSW KPWL LAGV VDVT SLSL LSDR KGLT RRER RELR RRTI LLLY YLLR SPFY DRFS EARI LFLL QLLA DHVP GVGL VTRP LMDY LPTW QKIY FYSW G
Gene ID:	9409
Uniprot:	Q9Y5Y5
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein, Peroxisome membrane
Calculated MW:	38kDa/39kDa
Observed MW:	39kDa

Synonyms:

PEX16, PBD8A, PBD8B

Background:

The protein encoded by this gene is an integral peroxisomal membrane protein. An inactivating nonsense mutation localized to this gene was observed in a patient with Zellweger syndrome of the complementation group CGD/CG9. Expression of this gene product morphologically and biochemically restores the formation of new peroxisomes, suggesting a role in peroxisome organization and biogenesis. Alternative splicing has been observed for this gene and two variants have been described.

UniProt Protein Function:	PEX16: Required for peroxisome membrane biogenesis. May play a role in early stages of peroxisome assembly. Can recruit other peroxisomal proteins, such as PEX3 and PMP34, to de novo peroxisomes derived from the endoplasmic reticulum (ER). May function as receptor for PEX3. Defects in PEX16 are the cause of peroxisome biogenesis disorder complementation group 9 (PBD-CG9); also known as PBD-CGD. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Defects in PEX16 are a cause of Zellweger syndrome (ZWS). ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. Belongs to the peroxin-16 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to peroxisomal membrane; membrane; peroxisomal membrane; peroxisome Molecular Function:protein binding; protein C-terminus binding Biological Process: ER-dependent peroxisome biogenesis; peroxisome membrane biogenesis; peroxisome organization and biogenesis; protein import into peroxisome matrix; protein import into peroxisome membrane; protein targeting to peroxisome; protein to membrane docking Disease: Peroxisome Biogenesis Disorder 8a (zellweger); Peroxisome Biogenesis Disorder 8b
NCBI Summary:	The protein encoded by this gene is an integral peroxisomal membrane protein. An inactivating nonsense mutation localized to this gene was observed in a patient with Zellweger syndrome of the complementation group CGD/CG9. Expression of this gene product morphologically and biochemically restores the formation of new peroxisomes, suggesting a role in peroxisome organization and biogenesis. Alternative splicing has been observed for this gene and two variants have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5Y5
NCBI GenInfo Identifier:	332278135
NCBI Gene ID:	9409
NCBI Accession:	Q9Y5Y5.2
UniProt Secondary Accession:	Q9Y5Y5,Q9BWB9,
UniProt Related Accession:	Q9Y5Y5
Molecular Weight:	39,270 Da
NCBI Full Name:	Peroxisomal membrane protein PEX16
NCBI Synonym Full Names:	peroxisomal biogenesis factor 16
NCBI Official Symbol:	PEX16
NCBI Official Synonym Symbols:	PBD8A; PBD8B
NCBI Protein Information:	peroxisomal biogenesis factor 16
UniProt Protein Name:	Peroxisomal membrane protein PEX16
UniProt Synonym Protein Names:	Peroxin-16; Peroxisomal biogenesis factor 16
Protein Family:	Peroxisomal membrane protein
UniProt Gene Name:	PEX16
UniProt Entry Name:	PEX16_HUMAN

Western blot analysis of extracts of various cell lines, using PEX16 antibody (CAB10387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

PEX16 Antibody (PACO11175)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)